Anti-cyclin A Antibody (E43.2) recommended for detection of cyclin A of mouse, rat and human origin by WB, IP and IF

Anti-cyclin A Antibody (E43.2): sc-53229

Anti-cyclin A Antibody (E43.2) is rated 4.0 out of 5 by 1.
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Datasheets

    • cyclin A Antibody (E43.2) is a mouse monoclonal IgG2a κ, cited in 3 publications, provided at 200 µg/ml
    • raised against human cyclin A
    • recommended for detection of cyclin A of mouse, rat and human origin by WB, IP and IF
    • See cyclin A (B-8): sc-271682 for cyclin A antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for cyclin A Antibody (E43.2) for WB applications. These reagents are now offered in bundles with cyclin A Antibody (E43.2) (see ordering information below).
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What blocking solution should I use with cyclin A (E43.2): sc-53229 monoclonal antibody for Western blot?

Asked by: AbPolly
Thank you for your question. We recommend blocking with Blotto (either TBS Blotto A: sc-2333 or TBS Blotto B: sc-2335). Our Western blot protocol can be found here: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-01
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Rated 4 out of 5 by from Good specificity and signal strength in K Good specificity and signal strength in K-562 and HuT 78 whole cell lysates. -SCBT QC
Date published: 2014-04-07
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