Anti-cyclin A Antibody (E23.1) recommended for detection of cyclin A of mouse, rat, human and bovine origin by WB, IP and IF

Anti-cyclin A Antibody (E23.1): sc-53228

Anti-cyclin A Antibody (E23.1) is rated 4.5 out of 5 by 2.
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Datasheets

    • cyclin A Antibody (E23.1) is a mouse monoclonal IgG2a κ, cited in 6 publications, provided at 200 µg/ml
    • raised against bovine cyclin A fusion protein
    • recommended for detection of cyclin A of mouse, rat, human and bovine origin by WB, IP and IF
    • See cyclin A (B-8): sc-271682 for cyclin A antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for cyclin A Antibody (E23.1) for WB applications. These reagents are now offered in bundles with cyclin A Antibody (E23.1) (see ordering information below).
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What is the recommended starting dilution for Western blot for sc-53228: cyclin A (E23.1) monoclonal antibody?

Asked by: Trav11
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 5 out of 5 by from very good With 1:3000 dilution by western blotting, the band is sharp and clear. This antibody is very good.
Date published: 2017-02-13
Rated 4 out of 5 by from Good strong specific band in HeLa Good strong specific band in HeLa, K-562 and HuT 78 whole cell lysates. -SCBT QC
Date published: 2014-05-26
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