Anti-cyclin A Antibody (BF683) recommended for detection of cyclin A of mouse, rat and human origin by WB, IP, IF, FCM, KA and ELISA

Anti-cyclin A Antibody (BF683): sc-239

Anti-cyclin A Antibody (BF683) is rated 4.5 out of 5 by 15.
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Datasheets

    • cyclin A Antibody (BF683) is a mouse monoclonal IgE, cited in 271 publications, provided at 200 µg/ml
    • raised against recombinant full length cyclin A of human origin
    • recommended for detection of cyclin A of mouse, rat and human origin by WB, IP, IF, FCM, KA and ELISA
    • available conjugated to agarose for IP; and to phycoerythrin for IF, IHC(P) and FCM
    • TransCruz reagent for Gel Supershift and ChIP applications (sc-239 X, 200 µg/0.1 ml)
    • See cyclin A (B-8): sc-271682 for cyclin A antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of cyclin A (BF683): sc-239.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for cyclin A Antibody (BF683). This work is in progress.
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When should the TransCruz reagent sc-293 X be used instead of the standard form sc-293?

Asked by: jenni
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support 14
Date published: 2017-01-09
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Rated 5 out of 5 by from WB Good results! A clear and strong band was showed in the WB.
Date published: 2020-11-15
Rated 1 out of 5 by from No Detection In INS-1 Rat Cells Tested by Western Blot (Li-Cor reagents) of rat insulinoma (INS-1) cell line. 60 ug loads were probed overnight, but no bands were detected. Presence of CcnA protein in this cell line was previously confirmed using discontinued product sc-596. Product has not been personally tested in human or mouse lines.
Date published: 2019-05-22
Rated 4 out of 5 by from The antibody is good The antibody was used to examine the protein of cyclin A in rat tissues by western blotting method. The bands were sepcific.
Date published: 2018-11-14
Rated 5 out of 5 by from Great product!! It's a great product for me to analyze Western blotting.
Date published: 2018-04-22
Rated 4 out of 5 by from a good antibody for WB This antibody generates a signal at the predicted position on the WB (1:500 dilution, 40ug HeLa extract). However, the signal intensity is not as good as that observed for sc-271682 in our hands.
Date published: 2017-09-18
Rated 4 out of 5 by from Works well! Very specific western blot results; strong clear signal
Date published: 2017-06-07
Rated 5 out of 5 by from WB I repeated the WB several times with this antibody, each time the band was clear
Date published: 2017-06-05
Rated 5 out of 5 by from Best cyclin A antibody Nice FCM data and strong western blot results !!!!
Date published: 2017-01-10
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