cyclin A Antibody (AT10.2)recommended for detection of cyclin A of mouse, rat and human origin by IP, IF and IHC(P)

cyclin A Antibody (AT10.2): sc-53227

cyclin A Antibody (AT10.2) is rated 5.0 out of 5 by 2.
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Datasheets

    • cyclin A Antibody (AT10.2) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against human recombinant cyclin A
    • recommended for detection of cyclin A of mouse, rat and human origin by IP, IF and IHC(P)
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See cyclin A (B-8): sc-271682 for cyclin A antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
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Can sc-53227: cyclin A (AT10.2) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Cweed
Thank you for your question. Yes, sc-53227: cyclin A (AT10.2) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by from Clean blot with single strong band at expected Clean blot with single strong band at expected size in K-562 and HuT 78 whole cell lysates. -SCBT QC
Date published: 2015-01-30
Rated 5 out of 5 by from Worked well for IF on PFA fixed human sample Worked well for IF on PFA fixed human sample -SCBT QC
Date published: 2014-04-01
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