Anti-cyclin A Antibody (25) recommended for detection of cyclin A of human origin by WB and IF

Anti-cyclin A Antibody (25): sc-136253

Anti-cyclin A Antibody (25) is rated 4.0 out of 5 by 1.
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Datasheets

    • cyclin A Antibody (25) is a mouse monoclonal IgG1, cited in 3 publications, provided at 50 µg/0.5 ml
    • raised against amino acids 26-144 of cyclin A of human origin
    • recommended for detection of cyclin A of human origin by WB and IF
    • See cyclin A (B-8): sc-271682 for cyclin A antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for cyclin A Antibody (25). This work is in progress.
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What is the recommended fixation method for immunofluorescence staining with cyclin A (25): sc-136253 monoclonal antibody?

Asked by: cjMara
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10ºC methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 4 out of 5 by from Good antibody for WB Good antibody for WB, strong band detected in A-431. -SCBT QC
Date published: 2013-05-05
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