Anti-CTH Antibody (A-2) recommended for detection of CTH isoforms 1 and 2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-CTH Antibody (A-2): sc-365381

Anti-CTH Antibody (A-2) is rated 5.0 out of 5 by 2.
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Datasheets

    • CTH Antibody (A-2) is a mouse monoclonal IgG2a κ, cited in 6 publications, provided at 200 µg/ml
    • raised against amino acids 61-227 mapping within an internal region of CTH of human origin
    • recommended for detection of CTH isoforms 1 and 2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • See CTH (F-1): sc-374249 for CTH antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for CTH Antibody (A-2). This work is in progress.
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I am using CTH (A-2): sc-365381 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Trav11
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Excellent immunoperoxidase cytoplasmic staining Excellent immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human kidney tissue. -SCBT QC
Date published: 2015-03-15
Rated 5 out of 5 by from Satisfactory Western Blot data of CTH expression Satisfactory Western Blot data of CTH expression in non-transfected 293T, mouse CTH transfected 293T, K-562 and HeLa whole cell lysates. -SCBT QC
Date published: 2014-11-22
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