CSN1 Antibody (F-8)recommended for detection of CSN1 of mouse, rat and human origin by WB, IP, IF and ELISA

CSN1 Antibody (F-8): sc-377134

CSN1 Antibody (F-8) is rated 5.0 out of 5 by 2.
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Datasheets

    • CSN1 Antibody (F-8) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 392-432 mapping near the C-terminus of CSN1 of human origin
    • recommended for detection of CSN1 of mouse, rat and human origin by WB, IP, IF and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
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What is the recommended fixation method for immunofluorescence staining with CSN1 (F-8): sc-377134 monoclonal antibody?

Asked by: Professor Griffin
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from Works well in Western Blot at 1:1000 dilution Works well in Western Blot at 1:1000 dilution
Date published: 2015-06-23
Rated 5 out of 5 by from Produced beautiful immunofluorescence nuclear Produced beautiful immunofluorescence nuclear and cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-02-18
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