CSB Antibody (D-7)recommended for detection of CSB of human origin by WB, IP, IF, IHC(P) and ELISA

CSB Antibody (D-7): sc-166042

CSB Antibody (D-7) is rated 5.0 out of 5 by 3.
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Datasheets

    • CSB Antibody (D-7) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 1-300 of CSB of human origin
    • CSB Antibody (D-7) is recommended for detection of CSB of human origin by WB, IP, IF, IHC(P) and ELISA
    • CSB Antibody (D-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CSB (D-7): sc-166042.
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Does this antibody recognize mouse CSB?

Asked by: Sarah Shuck
Thank you for your question. We have not yet tested this antibody, CSB (D-7): sc-166042, for reactivity with mouse samples and so this species reactivity is not currently recommended at this time. For detection of CSB of mouse origin, we would recommend an alternative antibody, CSB (E-6): sc-398022, which has been tested for reactivity with mouse, rat and human samples. A link to that product information is provided below as a reference. https://www.scbt.com/scbt/product/csb-antibody-e-6
Answered by: Technical Support
Date published: 2017-09-11

I am using CSB (D-7): sc-166042 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: jenni
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Great antibody Great results with CSB Antibody (D-7) by Western blot and IHCP
Date published: 2017-02-01
Rated 5 out of 5 by from Produced excellent immunoperoxidase nuclear Produced excellent immunoperoxidase nuclear staining in formalin fixed, paraffin-embedded human oral mucosa tissue. -SCBT QC
Date published: 2015-05-20
Rated 5 out of 5 by from Nice Western Blot data of CSB expression Nice Western Blot data of CSB expression in HeLa and BJAB nuclear extracts. -SCBT QC
Date published: 2014-07-21
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