Creatinine, anhydrousA substrate for creatininase

Creatinine, anhydrous (CAS 60-27-5)

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Synonym: 2-Amino-1-methyl-2-imidazolin-4-one, 2-Imino-1-methylimidazolidin-4-one, 2-Imino-N-methylhydantoin
Application: A substrate for creatininase
CAS Number: 60-27-5
Purity: ≥98%
Molecular Weight: 113.12
Molecular Formula: C4H7N3O
* Refer to Certificate of Analysis for lot specific data (including water content).
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Creatinine is a break-down product of creatine phosphate in muscle, and is usually produced at a fairly constant rate by the body. Creatinine levels in blood and urine may be used to calculate the creatinine clearance (CrCl), which reflects the glomerular filtration rate (GFR), an important clinical index of renal function.


1. Levey, Andrew S., et al., 2006. Using standardized serum creatinine values in the modification of diet in renal disease study equation for estimating glomerular filtration rate. Annals of internal medicine. 145(4): 247-54. PMID: 16908915
2. Allen, Patricia J., et al., 2012. Creatine metabolism and psychiatric disorders: Does creatine supplementation have therapeutic value? Neuroscience and biobehavioral reviews. 36(5): 1442-62. PMID: 22465051

Usage :
Long term storage: 2 years at -20°C (Powder); 6 months at -80°C (in DMSO)
Appearance :
Physical State :
Solubility :
Soluble in water (90 mg/ml at 20° C), alcohol (slightly), and ethanol (slightly). Insoluble in acetone, and chloroform.
Storage :
Store at room temperature
Melting Point :
295° C (lit.)(dec.)
Boiling Point :
255.49° C (Predicted)
Density :
1.09 g/cm3(Lit.)
Refractive Index :
n20D 1.65 (Predicted)
Ki Data :
PAT1: Ki= 15 mM (human)
pK Values :
pKa: 12.31 (Predicted), pKb: 4.83 (Predicted)
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
PubChem CID :
Merck Index :
14: 2569
MDL Number :
EC Number :
Beilstein Registry :

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Certificate of Analysis

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Asked by: ANGIEMC
Thank you for your question. Each lot of this chemical comes with a lot-specific Certificate Of Analysis which typically describes the percent purity as well as the method used to determine the purity. You may request a lot-specific Certificate Of Analysis on our website. Alternatively, please contact our Technical Service Department to inquire about the purity of our most recent lot. Please call us toll-free at (800) 457-3801 option 2, e-mail <>, or use the live chat on our website.
Answered by: Technical Support
Date published: 2018-01-31

What is the appearance of the compound?

Asked by: two2igm05
Thank you for your question. Creatinine, anhydrous, sc-207458, is a white or off- white crystalline powder.
Answered by: TechService7
Date published: 2017-01-27

Is the compound reagent grade?

Asked by: two2igm05
Thank you for your question. Yes, sc-207458 is reagent grade. If you have any further question or concerns, please feel free to contact our Technical Service department by calling 800-457-3801 option 2, emailing, or using the Live Chat function on our website.
Answered by: Tech Service 8
Date published: 2017-01-11
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