Anti-COUP-TF/EAR2 Antibody (F-11) recommended for detection of COUP-TFI, ARP-1 and EAR2 of mouse, rat and human origin by WB, IP, IF and ELISA

Anti-COUP-TF/EAR2 Antibody (F-11): sc-166941

Anti-COUP-TF/EAR2 Antibody (F-11) is rated 3.7 out of 5 by 3.
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Datasheets

    • Anti-COUP-TF/EAR2 Antibody (F-11) is a mouse monoclonal IgG1 (kappa light chain) COUP-TF/EAR2 antibody provided at 200 µg/ml
    • raised against amino acids 121-350 mapping within an internal region of EAR2 of human origin
    • Anti-COUP-TF/EAR2 Antibody (F-11) is recommended for detection of COUP-TFI, ARP-1 and EAR2 of mouse, rat and human origin by WB, IP, IF and ELISA
    • Anti-COUP-TF/EAR2 Antibody (F-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for ChIP application (sc-166941 X, 200 µg/0.1 ml)
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of COUP-TF/EAR2 (F-11): sc-166941.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for COUP-TF/EAR2 Antibody (F-11) for WB applications. These reagents are now offered in bundles with COUP-TF/EAR2 Antibody (F-11) (see ordering information below).
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What is the recommended fixation method for immunofluorescence staining with COUP-TF/EAR2 (F-11): sc-166941 monoclonal antibody?

Asked by: jerojero
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 1 out of 5 by from Not suitable for IF on embryonic mouse brain. IF was performed using the sample of this antibody on 4% PFA fixed embryonic mouse brain tissue (with antigen retrieval). No signal was detected.
Date published: 2020-10-20
Rated 5 out of 5 by from Exceptional immunofluorescence nuclear staining Exceptional immunofluorescence nuclear staining in formalin-fixed HepG2 cells. -SCBT QC
Date published: 2015-03-13
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