Coronin 1A Antibody (14.1)recommended for detection of Coronin 1A of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Coronin 1A Antibody (14.1): sc-100925

Coronin 1A Antibody (14.1) is rated 4.0 out of 5 by 7.
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Datasheets
    • Coronin 1A Antibody (14.1) is a mouse monoclonal IgG2a (kappa light chain) provided at 100 µg/ml
    • raised against a C-terminal region of Coronin 1A of human origin
    • recommended for detection of Coronin 1A of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Coronin 1A (14.1): sc-100925.
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Is Coronin 1A (14.1) monoclonal antibody suitable for IHC/IF?

Asked by: Ariamino
Thank you for your question. Yes, this mouse monoclonal antibody, Coronin 1A (14.1): sc-100925, is recommended for IHC(P) and IF. Immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, as well as immunofluorescence on fresh or frozen tissues or cells.
Answered by: Technical Support
Date published: 2017-10-25

I was using coronin1A antibody (sc-366201). I was planning to order it again but the problem is that the current list of coronin 1a antibodies is not having this one on this website. What should I do?

Asked by: Hriday S Pandey
Thank you for your question. Our rabbit polyclonal antibody, sc-366201: Coronin 1A (H-300) is now discontinued. As an alternative, we would recommend using our new mouse monoclonal antibody, Coronin 1A (14.1): sc-100925. https://www.scbt.com/scbt/product/coronin-1a-antibody-14-1
Answered by: Technical Support
Date published: 2017-09-28

I was using coronin1A antibody (sc-366201). I was planning to order it again but the problem is that the current list of coronin 1a antibodies on the website is not having this one. What should I do?

Asked by: Hriday
Thank you for your question. That rabbit polyclonal antibody, sc-366201, is now discontinued. We do offer a suggested replacement mouse monoclonal antibody, Coronin 1A (14.1): sc-100925. https://www.scbt.com/scbt/product/coronin-1a-antibody-14-1
Answered by: Technical Support
Date published: 2017-09-27

Can sc-100925: Coronin 1A (14.1) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: TinTin
Thank you for your question. Yes, sc-100925: Coronin 1A (14.1) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 2 out of 5 by from Very weak signal Used a 1:300 dilution for WB with 1- SUM159, 2- Caco-2, and 3- 293-T cell lysates. Detected a single band but the signal was very weak.
Date published: 2018-01-30
Rated 5 out of 5 by from WB for mouse brain was worked well. WB for mouse brain was worked well. Signal intensity was strong.
Date published: 2017-12-09
Rated 5 out of 5 by from Great IHC and WB antibody Observed specific stain and desired band with Coronin 1A Antibody (14.1)
Date published: 2017-01-23
Rated 4 out of 5 by from WB publishable data with NCI WB publishable data with NCI-H441 cell line and female BALB/c mice - SCBT Publication Review
Date published: 2015-04-29
Rated 4 out of 5 by from Good specificity in WB in K Good specificity in WB in K-562 whole cell lysate. -SCBT QC
Date published: 2015-04-13
Rated 4 out of 5 by from Robust signal in WB in human transfected Robust signal in WB in human transfected 293T whole cell lysates. -SCBT QC
Date published: 2015-01-15
Rated 4 out of 5 by from Produced good immunoperoxidase nuclear and Produced good immunoperoxidase nuclear and cytoplasmic staining in formalin-fixed, paraffin-embedded human lung tissue. -SCBT QC
Date published: 2014-02-28
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