Control siRNA-A consists of a scrambled sequence that will not lead to the specific degradation of any cellular message

Control siRNA-A

Control siRNA-A is rated 4.8 out of 5 by 42.
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Datasheets

    • resuspend to 66 µl, 10 µM; sufficient for 10-20 transfections
    • suitable as a negative control for experiments using targeted siRNA transfection
    • consists of a scrambled sequence that will not lead to the specific degradation of any cellular message
    • aliquot and store at -20°C
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CONTROL SIRNA-A は、非常に良くworkします。論文に記載したのですが配列を教えて頂いてもいいでしょうか?

Asked by: ken811
Thank you for your question. The sequences for control siRNA is proprietary, we cannot provide its sequences.
Answered by: Technical Support 12
Date published: 2021-08-31

Hi, for the siNC stock solution at 10µM. I would like to ask the unit, is it 10 µM/L? Thanks! 

Asked by: Han Gao
Thank you for your question. The unit µM refers to µmol per litre.
Answered by: Tech Support Europe
Date published: 2021-03-03

Hello, is the control siRNA Sc-37007 suitable to use in mouse immune and cancer cells? Thanks

Asked by: elibeli
Thank you for your question. Control siRNA-A sc-37007 is suitable as a negative control for targeted siRNA transfection experiments, including experiments with mouse immune and cancer cells. Its siRNA scrambled sequence will not lead to the specific degradation of any cellular message.
Answered by: Tech Support Europe
Date published: 2020-06-15

Can you share the resuspension protocol of control siRNA-A

Asked by: Lokesh91
Thank you for your question. Resuspend lyophilized control siRNA duplex in 66 μl of the RNAse-free water provided. Resuspension of the siRNA duplex in 66 μl of RNAse-free water makes a 10μM solution in a 10μM Tris-HCl, pH 8.0, 20 mM NaCl, 1mM EDTA buffered solution.
Answered by: Tech Service
Date published: 2021-10-05

does it work as a transfection control in mouse cells?

Asked by: Jáina
Thank you for your question. This is a negative control siRNA which contains a scrambled siRNA sequence that should not lead to the degradation of any gene product. It is not species-specific. Therefore, yes, you could use this as a negative control siRNA for transfections of mouse cells.
Answered by: Technical Support
Date published: 2019-06-16

We used control siRNA-A (sc-37007) as a scrambled siRNA in our project. It was very well. Our manuscript is under review and the reviewer asked us the sequence of this product. would you please give me some information about it. best regards. Reza

Asked by: Reza62
Thank you for your question. Please contact Technical Service for further assistance.
Answered by: SCBT Heidelberg Support
Date published: 2019-01-06

Hi, what's the difference between control siRNA A, B, C etc. and how do I choose the best for my siRNA experiments? Thanks in advance; All the best

Asked by: Fred69
Thank you for your question. The difference between the siRNA controls is the sequence: each consists of a different scrambled sequence. All these controls are however recommended and suitable as negative control for experiments with siRNA. Control siRNA A : sc-37007 is the most commonly used with currently more than 800 publications.
Answered by: SCBT Heidelberg Support
Date published: 2021-10-09

I have used siRNA-A (sc-37007) as a non-target control in my experiments. While preparing the figures I am facing a problem, siRNA-A has been used in nM, I need to convert it to ng/ml. Can you please state the M.W of siRNA-A? Thanks.

Asked by: Pradip Das
Thank you for your question. The molecular weight is 13191.24 g/mol.
Answered by: Technical Support
Date published: 2018-12-07
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Rated 2 out of 5 by from Alert to protein synthesis Attention with primary human fibroblasts silencing with siRNA-A and protein synthesis assays. Si-RNA-A blocks nascent protein synthesis (mitochondrial and cytosolic, measured by HPG) and increases eiF2a-P in my human primary fibroblasts.
Date published: 2021-10-09
Rated 5 out of 5 by from control siRNA sc-37007 control siRNA works well for knockdown experiments
Date published: 2021-02-13
Rated 5 out of 5 by from No issues Bought it few months back, works well, no off targeting observed.
Date published: 2021-01-13
Rated 5 out of 5 by from Good negative control This product did not significantly change the expression of target protein. It served as good control in comparison to the specific siRNA,
Date published: 2020-09-01
Rated 5 out of 5 by from Good control for siRNA transfection Can be used in any cell types without any toxicity to the cell
Date published: 2019-12-04
Rated 4 out of 5 by from Your Control siRNA-A work well Your Control siRNA-A work well and I was satisfied with results I received.
Date published: 2019-10-30
Rated 5 out of 5 by from Trusted product for HUVEC We have been using this product for many years on HUVEC, which can be trusted.
Date published: 2019-08-09
Rated 5 out of 5 by from Great siRNA! I brought it about 1 month ago. It works great! The knock down efficiency is high.
Date published: 2019-03-14
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