Control shRNA Plasmid-A
  • 20 µg, sufficient for up to 20 transfections
  • suitable as a negative control for experiments using targeted shRNA Plasmid DNA transfection
  • encodes of a scrambled shRNA sequence that will not lead to the specific degradation of any cellular message
  • After transfection, cells stably expressing the control shRNA may be isolated via puromycin selection
  • Store at 4° C for short term storage or -20° C for long term storage

Control shRNA Plasmid-A

Control shRNA Plasmid-A is rated 4.3 out of 5 by 3.
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Datasheets

    • 20 µg, sufficient for up to 20 transfections
    • suitable as a negative control for experiments using targeted shRNA Plasmid DNA transfection
    • encodes of a scrambled shRNA sequence that will not lead to the specific degradation of any cellular message
    • After transfection, cells stably expressing the control shRNA may be isolated via puromycin selection
    • Store at 4° C for short term storage or -20° C for long term storage
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Does it can work as control shRNA in both human and mouse cell line? Which competent cell strain is best? How to amplify the shRNA control plasimd?

Asked by: MINGLI
Thank you for your question. Yes, this is a suitable negative control for both human and mouse cell lines. It expresses a non-coding mismatched shRNA sequence that will not lead to the specific degradation of any cellular message. We do not recommend nor support further propagation of our shRNA plasmids in bacteria. They are provided conveniently as ready-to-use purified plasmid DNA, and are intended to be consumable reagents.
Answered by: Technical Support
Date published: 2020-03-11

Is the plasmid containing control shRNA (sc-108060) a lentiviral vector? Can I use it to produce lentiviral particles and infect my cells?

Asked by: Sam123456
Thank you for your question. Control shRNA Plasmid-A sc-108060 is based on the same lentiviral vector plasmid as our specific knockdown shRNA products. If you are looking for a control lentiviral particle product please check out Control shRNA Lentiviral Particles-A sc-108080 and fluorescent copGFP Control Lentiviral Particles sc-108084.
Answered by: Tech Support Europe
Date published: 2019-07-24

Can I have the details about the plasmid vector backbone (vector map) used in this construct? Thanks.

Asked by: Elith
Thank you for your question. Please contact our Technical Service Department and we can provide you with a basic vector map.
Answered by: Technical Support
Date published: 2020-08-18

I have bought thus product but I need information about the plasmid ackbone and the laboratory safety status of this product. Thank you for your help. Dr. Ali Sak

Asked by: alisak
Thank you for your question. Our lentiviral vectors do not pose a safety concern. The shRNA Plasmid DNA contains a self-inactivating biosafety feature. Lentiviral particles can be employed in standard Biosafety Level 2 tissue culture facilities (and should be treated with the same level of caution as with any other potentially infectious reagent). The Lentiviral Particles are replication-incompetent and are designed to self-inactivate after transduction and integration of the shRNA constructs into the genomic DNA of target cells. No. They are 3rd generation, biosafety level 2 particles. None of the HIV-1 genes (gag, pol, rev) will be present in the packaged viral genome, as they are expressed from packaging plasmids lacking packaging signal - therefore, the lentiviral particles generated are replication-incompetent. Deletion in the enhancer of U3 region of 3’LTR ensures self-inactivation of lentiviral construct after transduction and integration into genomic DNA of the target cells. We use the H1 and CMV promotors for the shRNA and puromycin genes respectively.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-30

Can I have the details about the plasmid vector backbone used in this construct?Thanks.

Asked by: nobutachi
Thank you for your question. This information is available upon request. We will e-mail you the vector map. Please let us know if you require any additional information.
Answered by: Technical Support
Date published: 2020-08-18

Sir/Madam, What is the difference between Control shRNA Plasmid-A, Control shRNA Plasmid-B and Control shRNA Plasmid-C? What is the promoter for these shRNA plasmids?

Asked by: Suresh B
Thank you for your question. The only difference between the Control shRNA Plasmids A, B and C are that they contain different scrambled shRNA sequences (that should not lead to the specific degradation of any cellular message). We offer more than one scrambled control shRNA sequence to provide multiple negative control options to researchers. The plasmid uses an H1 promoter for the shRNA.
Answered by: Technical Support
Date published: 2016-12-20
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Rated 5 out of 5 by from Great as a control for shRNA plasmids Great as a control for shRNA plasmids, did not affect the cellular physiology of the hypoxic response.
Date published: 2017-03-12
Rated 4 out of 5 by from good features I bought this six months ago and am so happy it worked well, sufficient for transfections and did not lead to the specific degradation of any cellular message.
Date published: 2016-10-30
Rated 4 out of 5 by from Used as a control in conjunction with SC Used as a control in conjunction with SC-35139-SH
Date published: 2015-04-15
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