Thank you for your question. Our lentiviral vectors do not pose a safety concern.
The shRNA Plasmid DNA contains a self-inactivating biosafety feature.
Lentiviral particles can be employed in standard Biosafety Level 2 tissue culture facilities (and should be treated with the same level of caution as with any other potentially infectious reagent). The Lentiviral Particles are replication-incompetent and are designed to self-inactivate after transduction and integration of the shRNA constructs into the genomic DNA of target cells. No.
They are 3rd generation, biosafety level 2 particles. None of the HIV-1 genes (gag, pol, rev) will be present in the packaged viral genome, as they are expressed from packaging plasmids lacking packaging signal - therefore, the lentiviral particles generated are replication-incompetent. Deletion in the enhancer of U3 region of 3’LTR ensures self-inactivation of lentiviral construct after transduction and integration into genomic DNA of the target cells. We use the H1 and CMV promotors for the shRNA and puromycin genes respectively.
Date published: 2018-12-30