Control Double Nickase Plasmid Control Double Nickase Plasmid is transfection-ready, purified plasma DNA that is provided as a negative control for Double Nickase Plasmids

Control Double Nickase Plasmid

Control Double Nickase Plasmid is rated 4.0 out of 5 by 2.
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Datasheets

    • 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
    • Control Double Nickase Plasmid is transfection-ready, purified plasma DNA that is provided as a negative control for Double Nickase Plasmids
    • Control Double Nickase Plasmid consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a unique, non-targeting 20 nt scramble guide RNA
    • One plasmid in the pair contains a puromycin-resistance gene; the other plasmid in the pair contains a GFP marker to visually confirm transfection
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Rated 3 out of 5 by from Easy to handle, but problems with the transfection The protocol related to the plasmid is really easy to perform, but we have the problem, that our cells are probable sensitive to GFP. So on the first step we get a efficiency of 10-20% GFP-positive cells, but they all die and only puro resistent cells survive.
Date published: 2018-02-16
Rated 5 out of 5 by from This control works very well and saw good knockout This control works very well and saw good knockout.
Date published: 2015-10-15
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