Control CRISPR/Cas9 Plasmid Control CRISPR/Cas9 Plasmid is a plasmid encoding the Cas9 nuclease and a non-specific 20 nt guide RNA. Used as a negative control for CRISPR/Cas9 KO Plasmids

Control CRISPR/Cas9 Plasmid

Control CRISPR/Cas9 Plasmid is rated 4.7 out of 5 by 3.
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Datasheets

    • 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
    • Control CRISPR/Cas9 Plasmid is a plasmid encoding the Cas9 nuclease and a non-specific 20 nt guide RNA. Used as a negative control for CRISPR/Cas9 KO Plasmids
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Como puedo saber que producto me favorece

Asked by: Tanque
Thank you for your question. Please contact our technical service team directly so we can assist you in selecting a suitable product for your experiments.
Answered by: Tech Support Europe
Date published: 2022-04-25

I have created KO cells with Santa Cruz CRISPR system, is there any way to have a CRISPR control that is RFP+ just like the KO cells?

Asked by: Iratxeum
Thank you for your question. All of our KO plasmids including controls are based on GFP expression for transfection control. We do not offer control CRISPR plasmids that encode RFP.
Answered by: Tech Support Europe
Date published: 2022-02-04

Which is the corresponding HDR plasmid of the CRISPR/Cas9 control plasmid (sc-418922)?

Asked by: Shirley H
Thank you for your question. The product is a negative control for CRISPR/Cas9 KO Plasmids, your sample can be positive control. 感谢您的咨询。 这个产品本身属阴性对照,阳性对照可以是您的实验同等条件下的样本。
Answered by: Ling Chen
Date published: 2021-12-15

Hello, I would like to know the difference between Plasmide Double Nickase (h) and (h2) NCAM/CD56. Thanks in advance

Asked by: Meroa
Thank you for your question. These two products have different gRNA sequences that target different sites within the same genetic sequence.
Answered by: Tech Support Europe
Date published: 2021-08-19

When does GFP expression usually peak? Using this vector in Jurkats and looking to sort GFP positive cells only.

Asked by: mikewhoneedshelp
Fore more information please contact Technical Service
Answered by: Tech Sales
Date published: 2021-05-13

Hi, we have received CRISPR plasmids as a lyophilized pellet by FEDEX. How long would it be stable if left at room temperature? We are currently suspending lab work due to the COVID-19 incidence, and nobody may be available in the lab. Thank you.

Asked by: Shugaku
Thank you for your question. The lyophilized CRISPR DNA plasmids are vacuum-sealed with a dessicant pack. They are shipped ambient and should be stable at room temperature for a while. We can not provide an exact number of days, but it should be fine to leave them at room temperature as shipped. For long term storage, we do recommend storing the lyophilized plasmids with the dessicant at 4C.
Answered by: Technical Support
Date published: 2020-04-03

Does this plasmid or the CRISPR/Cas9 Knockout Plasmids carry antibiotic resistance gene for maxi-prep?

Asked by: Matrix
Thank you for your question. Our CRISPR/Cas9 KO Plasmids are provided as transfection-ready purified plasmid DNA. We do not recommend nor support further preparation of the plasmids. They are intended to be a consumable reagent.
Answered by: Technical Support
Date published: 2021-01-28

Hi, I have question about SC-418922 negative control. How long express GFP in the cells after transfection? I want expand cell upto 10miliion. If I expand cells after transfection, does GFP keep express or decrease with cell division ?

Asked by: park
Thank you for your question. The GFP is not integrated into the host cell genome, it is expressed transiently from the plasmid while it is present in the cell. It will decrease with cell division. Please contact Technical Service by phone, (800)-457-3801 option 2, email <[email protected]>, or by live chat directly on our website, www.scbt.com if you have any further questions.
Answered by: Tech Service
Date published: 2020-02-24
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Rated 4 out of 5 by from Good Product It worked very well in our hands. Good product for CRISPRs experiments.
Date published: 2020-07-03
Rated 5 out of 5 by from We are very satisfied with this product We are very satisfied with this product. We used the plasmid as control for knock-out of ATF-2 in human colorectal adenocarcinoma cell line Colo201 and had no effect on ATF-2.
Date published: 2015-10-09
Rated 5 out of 5 by from The CRISPR negative control demonstrated The CRISPR negative control demonstrated that the non-specific gRNAs did not effect E2F-1 and GAPDH expression in transfected HEK 293T whole cell lysates. -SCBT QC
Date published: 2015-03-24
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