ColcemidA cell synchronizing, anti-tubulin agent

Colcemid (CAS 477-30-5)

Colcemid | CAS 477-30-5 is rated 5.0 out of 5 by 1.
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Synonym: Demecolcine
Application: A cell synchronizing, anti-tubulin agent
CAS Number: 477-30-5
Purity: ≥97%
Molecular Weight: 371.43
Molecular Formula: C21H25NO5
Supplemental Information: This is classified as a Dangerous Good for transport and may be subject to additional shipping charges.
* Refer to Certificate of Analysis for lot specific data (including water content).
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Colcemid, a cell sychronizing agent, has been reported to induce coordination of nuclear and cytoplasmic events during cell growth. At different concentrations, this compound has been shown to disrupt cell cycle integration and increase cell size. Colcemid depolarizes microtubules and inactivates spindle fiber formation, thus arresting cells in metaphase and allowing cell harvest and karyotyping to be performed.


References

1. Sherwood, S.W., et al. 1994. Exp. Cell Res. 215: 373-379. PMID: 7982475
2. Kanda, R., et al. 1994. J. Radiat. Res. 35: 41-47. PMID: 8057270
3. Urbani, L., et al. 1995. Exp. Cell Res. 219: 159-168. PMID: 7628532

Appearance :
Powder
Physical State :
Solid
Solubility :
Soluble in ethanol, DMSO, chloroform (10 mg/ml), water, ether, benzene, and acidified water.
Storage :
Store at room temperature
Melting Point :
186° C
Boiling Point :
~625.5° C at 760 mmHg (Predicted)
Density :
~1.2 g/cm3 (Predicted)
Refractive Index :
n20D 1.58 (Predicted)
IC50 :
T-cell specific antigen receptor-induced NF-kB activation: IC50 = <160 nM (HEK-293T cell line)
pK Values :
pKa: 6.31, pKb: 8.48 (Predicted)
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
RTECS :
GH0800000
PubChem CID :
220401
Merck Index :
14: 2887
MDL Number :
MFCD00075459
EC Number :
207-514-6
Beilstein Registry :
2822892
SMILES :
CN[C@H]1CCC2=CC(=C(C(=C2C3=CC=C(C(=O)C=C13)OC)OC)OC)OC

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Certificate of Analysis

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Colcemid  Product Citations

See how others have used Colcemid. Click on the entry to view the PubMed entry .

Citations 1 to 2 of 2 total

PMID: # 30296733  Khalil, AM.|Abu Samrah, HM.| et al. 2018. Res. Vet. Sci. 120: 94-100.

PMID: # 25208094  Falck Miniotis, M. et al. 2014. PloS one. 9: e106546.

Citations 1 to 2 of 2 total

Can this product be stored in solution?

Asked by: hawkeye11
This chemical, Colcemid (CAS 477-30-5), is stable when stored in Chloroform.
Answered by: Chemical Support 1
Date published: 2017-03-27
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Rated 5 out of 5 by from Lee Lee, J. et al. (PubMed 25982990) examined the effects of Colcemid, a cell synchronizing, anti-tubulin agent, treatment during oocyte in vitro maturation (IVM) and embryonic development after parthenogenetic activation (PA) and somatic-cell nucleus transfer (SCNT) in pigs. Their results suggest that the presence of Colcemid during the early stage of IVM stimulates preimplantation development of PA and SCNT porcine embryos by improving the cytoplasmic microenvironment. -SCBT Publication Review
Date published: 2015-07-13
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