coilin Antibody (F-7)recommended for detection of coilin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

coilin Antibody (F-7): sc-55594

coilin Antibody (F-7) is rated 4.9 out of 5 by 13.
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Datasheets

    • coilin Antibody (F-7) is a mouse monoclonal IgG1 (lambda light chain) provided at 200 µg/ml
    • raised against amino acids 277-576 mapping at the C-terminus of coilin of human origin
    • coilin Antibody (F-7) is recommended for detection of coilin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • coilin Antibody (F-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGλ BP-HRP (mouse IgGλ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of coilin (F-7): sc-55594.
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I am using coilin (F-7): sc-55594 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: TinTin
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24

Hello, I wanted to ask if there would be any recommendations on using triton to fix the cells?

Asked by: ps95
Thank you for your inquiry. If you are using formalin to fix your cells, then we do recommend using Triton to permeabilize your cells. After fixation, wash in 3 changes of PBS for 5 minutes each. Then, add a solution of .01% Triton X-100 in PBS and incubate for 3 minutes. Wash in 3 changes of PBS for 5 minutes each. Then proceed with blocking. A link to our recommended protocol for immunofluorescence cell staining is provided for your refernece. https://www.scbt.com/scbt/resources/protocols/immunoflourescence-cell-staining
Answered by: Technical Support
Date published: 2016-11-08
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Rated 5 out of 5 by from HCT116細胞での免疫染色とウェスタンブロットできました。 HCT116細胞の免疫染色とウェスタンブロットできました。免疫染色に関しては、HeLa細胞とMCF7細胞でOKでした。なお、ウェスタンブロットは、Pdeltaよりも非特異的なバンドが少ない印象でした。
Date published: 2019-04-17
Rated 5 out of 5 by from strong band seen in WB This antibody detect the target coilin in HeLa cell lysate, with 80KD specific band. It work well in western blot at 1:1000.
Date published: 2017-11-13
Rated 4 out of 5 by from Marche bien en IF Anticorps marche super bien, testé au 1:100e en IF sur cellules fixées au PFA3% 15min
Date published: 2017-11-07
Rated 5 out of 5 by from specific bands This antibody detect the target coilin in human liver tumor tissue extracts .It shows specific bands at 80kda.I suggest controled the dilution between 1:400 to 1:1000.Futher diluted rate such as 1:1500 shows very weak signal.
Date published: 2017-09-09
Rated 5 out of 5 by from used for WB This antibody titer is very high, it suitable for WB detection of coilin protein, .1:800 dilued the antibody can got strong signal,very cost-effective antibody.
Date published: 2017-07-22
Rated 5 out of 5 by from excellent antibody for IF This antibody works very well for IF (a very strong signal even when used at 1:750 dilution). It also works for WB at 1:500 dilution (I suspect the signal would be stronger at lower dilutions).
Date published: 2017-07-05
Rated 5 out of 5 by from strong band seen Great band obtained in WB on human intervertebral disc specimers,very strong band seen.
Date published: 2017-05-24
Rated 5 out of 5 by from work well The antibody produced some staining in the nuculear of the cardiac cells in rat tissue. The antibody seems to work well .
Date published: 2017-05-13
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