coilin Antibody (B-7)recommended for detection of coilin of mouse, rat and human origin by WB, IP, IF and ELISA

coilin Antibody (B-7): sc-55595

coilin Antibody (B-7) is rated 5.0 out of 5 by 2.
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Datasheets

    • coilin Antibody (B-7) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 277-576 mapping at the C-terminus of coilin of human origin
    • recommended for detection of coilin of mouse, rat and human origin by WB, IP, IF and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
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What is the recommended fixation method for immunofluorescence staining with coilin (B-7): sc-55595 monoclonal antibody?

Asked by: Germaine
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from Clean bands in Western blot analysis of coilin Clean bands in Western blot analysis of coilin expression in MOLT-4 and Jurkat nuclear extracts. -SCBT QC
Date published: 2015-03-14
Rated 5 out of 5 by from Excellent immunofluorescence nuclear staining Excellent immunofluorescence nuclear staining in formalin-fixed HepG2 cells. -SCBT QC
Date published: 2015-02-17
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