Anti-Clusterin-alpha Antibody (78E) recommended for detection of Clusterin-α of human origin by WB, IP, IF and IHC(P)

Anti-Clusterin-α Antibody (78E): sc-32264

Anti-Clusterin-α Antibody (78E) is rated 5.0 out of 5 by 3.
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Datasheets

    • Clusterin-α Antibody (78E) is a mouse monoclonal IgG1, cited in 1 publications, provided at 200 µg/ml
    • raised against native Clusterin purified from human serum
    • recommended for detection of Clusterin-α of human origin by WB, IP, IF and IHC(P)
    • See Clusterin-α (B-5): sc-5289 for Clusterin-α antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Clusterin-α Antibody (78E). This work is in progress.
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Can Clusterin-α (78E): sc-32264 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: AbPolly
Thank you for your question. Yes, Clusterin-α (78E): sc-32264 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-28
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Rated 5 out of 5 by from Strong clear band in human transfected 293T Strong clear band in human transfected 293T whole cell lysates. -SCBT QC
Date published: 2015-05-11
Rated 5 out of 5 by from Robust band observed in western blot analysis Robust band observed in western blot analysis of Clusterin-a expression in human peripheral blood lysate. -SCBT QC
Date published: 2015-05-03
Rated 5 out of 5 by from Produced superb immunoperoxidase membrane Produced superb immunoperoxidase membrane staining in formalin fixed, paraffin-embedded human tonsil tissue. -SCBT QC
Date published: 2015-01-06
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