Chromeo™ 546 streptavidin is conjugated to Chromeo™ 546, a bright, red fluorescent label for enabling detection in immunofluorescence, high content screening, ELISA and FRET applications, as well as in flow cytometry. It exhibits superior luminescence properties, a broad Stokes shift, stability towards photobleaching and pH, and low cell toxicity. Chromeo™ 546 is compatible with various excitation sources including mercury arc, tungsten and xenon arc lamps, the 544nm line of the Helium-Neon laser and the 532nm green laser line. It is comparable to CruzFluor™ 555 dyes. Chromeo™ 546 streptavidin has a molar extinction coefficient of 96,800M-1cm-1 and its absorption and emission wavelength is 553nm and 567.5nm, respectively. It has a quantum yield of 15% and a dye to protein ratio 1.0-2.5.
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