Chromeo 546 streptavidin A bright, red fluorescent labeled conjugate for bioanalysis.

Chromeo™ 546 streptavidin

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Application: Chromeo™ 546 streptavidin is a bright, red fluorescent labeled conjugate for bioanalysis
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data (including water content).

Chromeo™ 546 streptavidin is conjugated to Chromeo™ 546, a bright, red fluorescent label for enabling detection in immunofluorescence, high content screening, ELISA and FRET applications, as well as in flow cytometry. It exhibits superior luminescence properties, a broad Stokes shift, stability towards photobleaching and pH, and low cell toxicity. Chromeo™ 546 is compatible with various excitation sources including mercury arc, tungsten and xenon arc lamps, the 544nm line of the Helium-Neon laser and the 532nm green laser line. It is comparable to CruzFluor™ 555 dyes. Chromeo™ 546 streptavidin has a molar extinction coefficient of 96,800M-1cm-1 and its absorption and emission wavelength is 553nm and 567.5nm, respectively. It has a quantum yield of 15% and a dye to protein ratio 1.0-2.5.


References

1. Wetzl, B. et al. 2003. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 793(1): 83-92. PMID: 12880856<br> 2. Gruber, M. et al. 2005. J Fluoresc. 15(3): 207-214. PMID: 15986147<br> 3. Cang, H. et al. 2011. Nature. 469(7330): 385-388. PMID: 21248848<br>

Formulation :
1 mg/ml PBS containing 0.2% blocking reagent and 0.09% Azide
Physical State :
Liquid
Storage :
Store at 4° C
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Download SDS (MSDS)

Certificate of Analysis

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