Chromeo™ 494 alkyne is conjugated to Chromeo™ 494, which is a unique red fluorescent label that is excitable at a wavelength of 488nm by argon laser, LED, xenon-arc or tungsten-halogen lamps. It has a very large Stokes shift of 134nm, making it an ideal partner for multiplexing in immunofluorescence experiments. Chromeo™ 494 serves as a bright label for antibodies and other biomolecules, enabling detection in immunofluorescence, high content screening, ELISA and FRET applications, as well as in flow cytometry. It is comparable to CruzFluor™ 488 and CruzFluor sm™ dyes. Chromeo™ 494 alkyne has a molar extinction coefficient of 55,000M-1cm-1 and its absorption and emission wavelength is 494nm and 628nm, respectively.
1. Kato, Y. et al. 2011. Dev. Genes Evol. 220(11-12): 337-345. PMID: 21327957