CHMP4B Double Nickase Plasmid (h): sc-401802-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CHMP4B Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CHMP4B Double Nickase Plasmid (h) and CHMP4B Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CHMP4B. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CHMP4B Double Nickase Plasmid (h) | sc-401802-NIC | 20 µg | $410.00 | |||
CHMP4B Double Nickase Plasmid (h2) | sc-401802-NIC-2 | 20 µg | $410.00 |
CHMP4B (charged multivesicular body protein 4B) is a core component of the ESCRT-III complex that drives membrane remodeling events required for endosomal sorting and multivesicular body biogenesis. By polymerizing on membranes and coordinating with VPS4 ATPase activity, CHMP4B supports cargo sequestration, intraluminal vesicle formation, and the terminal steps of cytokinetic abscission, linking it to pathways controlling receptor downregulation and membrane scission. CHMP4B-dependent ESCRT function also contributes to plasma membrane repair and aspects of autophagy and lysosomal homeostasis through endolysosomal trafficking. Genetic perturbation of ESCRT-III components, including CHMP4B, is associated with defects in cell division and proteostasis and has been implicated in neurodegenerative and ocular disease contexts as well as altered signaling in cancer-relevant endocytic networks.
CHMP4B Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CHMP4B locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CHMP4B. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CHMP4B function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CHMP4B-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.