CHMP3 Antibody (F-1)recommended for detection of CHMP3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

CHMP3 Antibody (F-1): sc-166361

CHMP3 Antibody (F-1) is rated 5.0 out of 5 by 3.
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Datasheets

    • CHMP3 Antibody (F-1) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 1-222 representing full length CHMP3 of human origin
    • CHMP3 Antibody (F-1) is recommended for detection of CHMP3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • CHMP3 Antibody (F-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CHMP3 (F-1): sc-166361.
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I am using CHMP3 (F-1): sc-166361 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Trav11
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-25
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Rated 5 out of 5 by from Strong band of interest observed in WB in non Strong band of interest observed in WB in non-transfected and mouse CHMP3 transfected 293T whole cell lysates. -SCBT QC
Date published: 2015-05-20
Rated 5 out of 5 by from Produced beautiful immunoperoxidase cytoplasmic Produced beautiful immunoperoxidase cytoplasmic and membrane staining in formalin fixed, paraffin-embedded human lung tissue. -SCBT QC
Date published: 2015-04-20
Rated 5 out of 5 by from Positive result in WB in Hep G2 whole cell lysate Positive result in WB in Hep G2 whole cell lysate. -SCBT QC
Date published: 2015-01-04
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