Chk1 Antibody (G-4)recommended for detection of Chk1 of mouse, rat and human origin by WB, IP, IF, IHC(P), FCM and ELISA

Chk1 Antibody (G-4): sc-8408

Chk1 Antibody (G-4) is rated 4.8 out of 5 by 33.
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Datasheets

    • Chk1 Antibody (G-4) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 1-476 representing full length Chk1 of human origin
    • Chk1 Antibody (G-4) is recommended for detection of Chk1 of mouse, rat and human origin by WB, IP, IF, IHC(P), FCM and ELISA
    • Chk1 Antibody (G-4) is available conjugated to agarose for IP; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Chk1 (G-4): sc-8408.
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Does this antibody recognize hamster total Chk1?

Asked by: DrAKA
Thank you for your question. We do not have any data or product citations using this antibody, Chk1 (G-4): sc-8408, for detection of Chk1 in hamster. The antibody is raised against full-length Chk1 of human origin, and based on a predicted protein sequence for Chk1 in hamster (accession number: XP_003511081) this sequence shares 95% sequence identity with the hamster protein. That is a significant, and if the predicted sequence is correct, then there is a very good chance the antibody may also cross-react with hamster.
Answered by: Technical Support
Date published: 2017-09-07

What do you recommend for a positive control for Western blot?

Asked by: Dr Ninau Qelp
We have several recommended positive controls that can be used with Chk1 Antibody (G-4): sc-8408 for Western blot (WB). MDA-MB-231 Cell Lysate: sc-2232 HCT-116 Whole Cell Lysate: sc-364175 PANC-1 Whole Cell Lysate: sc-364380 HeLa Whole Cell Lysate: sc-2200 NIH/3T3 Whole Cell Lysate: sc-2210
Answered by: Tech Service 2
Date published: 2016-12-16

Can Chk1 (G-4) be used for chicken DT40 cell line?

Asked by: Dr Ninau Qelp
Yes! We have several publications referencing Chk1 Antibody (G-4): sc-8408 being used with chicken DT40 samples. Please see PMID: 26039276, 21765472, 17575048, 16619043 and 12554671.
Answered by: Tech Service 2
Date published: 2016-12-16
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Rated 5 out of 5 by from excellent Only single band. Very good antibody. .
Date published: 2019-12-03
Rated 5 out of 5 by from good product Good antibody with clear background, one single band.
Date published: 2018-11-02
Rated 5 out of 5 by from Excelent I am happy to use this for WB and intending to buy in future.
Date published: 2018-03-12
Rated 5 out of 5 by from Review for Chk1 antibody We purchased this antibody from Santa Cruz based on the previously published reference. On our hand, this antibody works fine, we get the results as we predicated. The quality of this antibody is good!
Date published: 2018-03-01
Rated 4 out of 5 by from great features I applied for the samples several days ago and they gave it to me immediately. very good and sensitive.
Date published: 2018-01-31
Rated 5 out of 5 by from Great antibody This works very well in my experiments. I could get clear bands using 1:1000 dilution and no other non-specific bands.
Date published: 2018-01-08
Rated 5 out of 5 by from Great antibody for western blot The batch of antibody I have worked well on BJAB, HeLa, MCF7, NIH/3T3, Jurkat and K562 total cell extracts for western Blot. The band is specific. The antibody was diluted 250 times into 5% BSA, TBST and incubated over-night at 4°C.
Date published: 2018-01-04
Rated 5 out of 5 by from excellent performance in wb I have performed wb on rat primary cardiomyocyte and mouse heart with sharp bands!
Date published: 2017-09-14
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