Chk1 Antibody (2G11D5)recommended for detection of Chk1 of mouse, rat and human origin by WB and IP

Chk1 Antibody (2G11D5): sc-56288

Chk1 Antibody (2G11D5) is rated 5.0 out of 5 by 4.
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Datasheets

    • Chk1 Antibody (2G11D5) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against Ni-NTA purified recombinant Chk1 of human origin
    • recommended for detection of Chk1 of mouse, rat and human origin by WB and IP
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See Chk1 (G-4): sc-8408 for Chk1 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
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Are there any issues with using sc-56288: Chk1 (2G11D5) mouse monoclonal primary antibody on mouse samples?

Asked by: Germaine
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent,we recommend using a directly conjugated primary antibody. Please contact our special orders department for pricing and availability of sc-56288 conjugates.
Answered by: Technical Support
Date published: 2017-03-07
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Rated 5 out of 5 by from Robust signal in WB in NIH/3T3 and HeLa whole Robust signal in WB in NIH/3T3 and HeLa whole cell lysates under reducing and non-reducing conditions. -SCBT QC
Date published: 2015-04-10
Rated 5 out of 5 by from Strong band in WB in HeLa Strong band in WB in HeLa, K-562 and NIH/3T3 whole cell lysates. -SCBT QC
Date published: 2015-02-18
Rated 5 out of 5 by from Positive signal in WB in 293T mouse transfected Positive signal in WB in 293T mouse transfected and K-562 whole cell lysates. -SCBT QC
Date published: 2015-02-05
Rated 5 out of 5 by from Good publishable data in WB in human lymphoblastoid Good publishable data in WB in human lymphoblastoid cells, fibroblasts. -SCBT Publication Review
Date published: 2013-04-03
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