CHD6 Antibody (A-5)recommended for detection of CHD6 of mouse, rat and human origin by WB, IP, IF and ELISA

CHD6 Antibody (A-5): sc-393421

CHD6 Antibody (A-5) is rated 5.0 out of 5 by 1.
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Datasheets

    • CHD6 Antibody (A-5) is a mouse monoclonal mouse IgG1 κ (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 243-320 mapping near the N-terminus of CHD6 of human origin
    • recommended for detection of CHD6 of mouse, rat and human origin by WB, IP, IF and ELISA
    • TransCruz reagent for ChIP application (sc-393421 X, 200 µg/0.1 ml)
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
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How much sample do you recommend loading on the gel for Western blot detection using CHD6 (A-5): sc-393421?

Asked by: Trav11
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from Very good Western blot data of CHD6 expression Very good Western blot data of CHD6 expression in Y79 whole cell lysate. -SCBT QC
Date published: 2014-06-17
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