CEPT1 Double Nickase Plasmid (m): sc-430281-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CEPT1 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CEPT1 Double Nickase Plasmid (m) and CEPT1 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Cept1. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CEPT1 Double Nickase Plasmid (m) | sc-430281-NIC | 20 µg | $410.00 | |||
CEPT1 Double Nickase Plasmid (m2) | sc-430281-NIC-2 | 20 µg | $410.00 |
Mouse Cept1 encodes choline/ethanolamine phosphotransferase 1 (CEPT1), an endoplasmic reticulum membrane enzyme that catalyzes the final step of the Kennedy pathway to generate phosphatidylcholine and phosphatidylethanolamine from CDP-choline or CDP-ethanolamine and diacylglycerol. By controlling major glycerophospholipid pools, CEPT1 supports membrane biogenesis, organelle homeostasis, and lipid-dependent signaling processes that influence proliferation, differentiation, and cellular stress responses. Perturbation of phospholipid synthesis and remodeling is broadly relevant to metabolic dysfunction, neurobiology, and oncogenic cell-state transitions, making Cept1 a useful node for interrogating lipid-driven phenotypes in mammalian cells. CEPT1 function is also connected to pathways governing ER integrity and secretory capacity, linking lipid availability to proteostasis and membrane trafficking.
CEPT1 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Cept1 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Cept1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Cept1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Cept1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.