Anti-CENP-B Antibody (F-4) recommended for detection of CENP-B of mouse, rat and human origin by WB, IP, IF and ELISA

Anti-CENP-B Antibody (F-4): sc-376283

Anti-CENP-B Antibody (F-4) is rated 4.4 out of 5 by 5.
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Datasheets

    • Anti-CENP-B Antibody (F-4) is a mouse monoclonal IgG1 κ CENP-B antibody, cited in 5 publications, provided at 200 µg/ml
    • raised against amino acids 535-599 mapping at the C-terminus of CENP-B of human origin
    • Anti-CENP-B Antibody (F-4) is recommended for detection of CENP-B of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine and bovine
    • Anti-CENP-B Antibody (F-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CENP-B (F-4): sc-376283.
    • m-IgG1 BP-HRP is the preferred secondary detection reagent for CENP-B Antibody (F-4) for WB applications. This reagent is now offered in a bundle with CENP-B Antibody (F-4) (see ordering information below).
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What is the recommended fixation method for immunofluorescence staining with CENP-B (F-4): sc-376283 monoclonal antibody?

Asked by: Germaine
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2018-06-26
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Rated 5 out of 5 by from Great antibody for WB! It works really well in WB. It gives a strong band at 70 kDa.Dilution 1:3000
Date published: 2017-10-19
Rated 2 out of 5 by from there is no signal in mouse oocyte I have tried this antibody in mouse MI and MII oocyte using IF at 1:50, but there was no signal detected. I had added the photo in another antibody's review.
Date published: 2017-07-24
Rated 5 out of 5 by from Immunofluorescence Staining in human cell line showed very nice results!
Date published: 2017-02-18
Rated 5 out of 5 by from Good specificity in WB in HeLa whole cell Good specificity in WB in HeLa whole cell lysate and Jurkat nuclear extract. -SCBT QC
Date published: 2015-05-07
Rated 5 out of 5 by from Robust signal in WB in human transfected Robust signal in WB in human transfected 293T whole cell lysates. -SCBT QC
Date published: 2014-12-19
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