Anti-CENP-B Antibody (C-10) recommended for detection of CENP-B of mouse, rat and human origin by WB, IP, IF and ELISA

Anti-CENP-B Antibody (C-10): sc-376392

Anti-CENP-B Antibody (C-10) is rated 5.0 out of 5 by 3.
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Datasheets

    • CENP-B Antibody (C-10) is a mouse monoclonal IgG2a κ, cited in 3 publications, provided at 200 µg/ml
    • raised against amino acids 535-599 mapping at the C-terminus of CENP-B of human origin
    • Anti-CENP-B Antibody (C-10) is recommended for detection of CENP-B of mouse, rat and human origin by WB, IP, IF and ELISA
    • Anti-CENP-B Antibody (C-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CENP-B (C-10): sc-376392.
    • m-IgGκ BP-HRP is the preferred secondary detection reagent for CENP-B Antibody (C-10) for WB applications. This reagent is now offered in a bundle with CENP-B Antibody (C-10) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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What does it mean if my test results are 149 for CENP A and 186 for CENP B?

Asked by: Jennylee
Thank you for your question. It would be helpful if you could call us directly at 800-457-3801 ext. 1, allowing for a more interactive discussion of this and other related questions.
Answered by: Blake J
Date published: 2021-08-25

What is the recommended fixation method for immunofluorescence staining with CENP-B (C-10): sc-376392 monoclonal antibody?

Asked by: Germaine
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Good antibody for IF The CENP-B (C-10); sc-376392 performs well for IF showing nuclear localized staining pattern.
Date published: 2017-01-19
Rated 5 out of 5 by from Produced great immunofluorescence nuclear Produced great immunofluorescence nuclear staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-04-24
Rated 5 out of 5 by from Satisfactory Western Blot data of CENP Satisfactory Western Blot data of CENP-B expression in non-transfected and human CENP-B transfected 293T whole cell lysates. -SCBT QC
Date published: 2013-12-30
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