CENP-B Antibody (C-10) is a mouse monoclonal IgG2a κ, cited in 3 publications, provided at 200 µg/ml
raised against amino acids 535-599 mapping at the C-terminus of CENP-B of human origin
Anti-CENP-B Antibody (C-10) is recommended for detection of CENP-B of mouse, rat and human origin by WB, IP, IF and ELISA
Anti-CENP-B Antibody (C-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CENP-B (C-10): sc-376392.
m-IgGκ BP-HRP is the preferred secondary detection reagent for CENP-B Antibody (C-10) for WB applications. This reagent is now offered in a bundle with CENP-B Antibody (C-10) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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A replicated chromosome includes two kinetochores that control chromosome segregation during mitosis. Both centromere proteins CENP-B and CENP-H are contained in the centromeric heterochromatin between kinetochores, and are involved in maintaining sister chromatid cohesion. The highly dispersed CENP-B promotes and maintains the joining of DNA satellites in the centromere. CENP-B targets centromeric a-DNA and protects it from digestion by nucleases as well as preventing DNAse or restriction enzyme digestion from affecting the morphology of centromeres. CENP-H contains a coiled-coil structure and a nuclear localization signal. CENP-H is specifically and constitutively localized to kinetochores and plays a role in the organization and function of kinetochores throughout the cell cycle.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
What does it mean if my test results are 149 for CENP A and 186 for CENP B?
Asked by: Jennylee
Thank you for your question. It would be helpful if you could call us directly at 800-457-3801 ext. 1, allowing for a more interactive discussion of this and other related questions.
Answered by: Blake J
Date published: 2021-08-25
What is the recommended fixation method for immunofluorescence staining with CENP-B (C-10): sc-376392 monoclonal antibody?
Asked by: Germaine
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here:
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