Anti-Cdt2 Antibody (B-8) recommended for detection of Cdt2 of human origin by WB, IP, IF and ELISA

Anti-Cdt2 Antibody (B-8): sc-166735

Anti-Cdt2 Antibody (B-8) is rated 5.0 out of 5 by 3.
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Datasheets

    • Anti-Cdt2 Antibody (B-8) is a mouse monoclonal IgG1 κ Cdt2 antibody, cited in 1 publications, provided at 200 µg/ml
    • raised against amino acids 1-240 mapping at the N-terminus of Cdt2 of human origin
    • Cdt2 Antibody (B-8) is recommended for detection of Cdt2 of human origin by WB, IP, IF and ELISA
    • Anti-Cdt2 Antibody (B-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Cdt2 (B-8): sc-166735.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Cdt2 Antibody (B-8) for WB applications. These reagents are now offered in bundles with Cdt2 Antibody (B-8) (see ordering information below).
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What is the recommended starting dilution for Western blot for sc-166735: Cdt2 (B-8) monoclonal antibody?

Asked by: AbPolly
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 5 out of 5 by from Good Antibody for Westerns! Highly specific and gives clean Westerns. Signal is a bit weak, will need to optimize further.
Date published: 2018-01-31
Rated 5 out of 5 by from Specific signal in response to drug treatment. Got nice signal at 1:300 in 2%NFDM. Molecular weight of protein was a bit higher than expected; runs above 98 KDa MWM.
Date published: 2017-04-07
Rated 5 out of 5 by from Strong band in WB in human transfected 293T Strong band in WB in human transfected 293T whole cell lysates. -SCBT QC
Date published: 2013-10-05
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