Anti-Cdt1 Antibody (F-6) recommended for detection of Cdt1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-Cdt1 Antibody (F-6): sc-365305

Anti-Cdt1 Antibody (F-6) is rated 4.5 out of 5 by 10.
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Datasheets

    • Anti-Cdt1 Antibody (F-6) is a mouse monoclonal IgG2b κ Cdt1 antibody, cited in 13 publications, provided at 200 µg/ml
    • raised against amino acids 247-546 mapping at the C-terminus of Cdt1 of human origin
    • Anti-Cdt1 Antibody (F-6) is recommended for detection of Cdt1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-Cdt1 Antibody (F-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Cdt1 (F-6): sc-365305.
    • m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Cdt1 Antibody (F-6) for WB and IHC(P) applications. These reagents are now offered in bundles with Cdt1 Antibody (F-6) (see ordering information below).
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Is this antibody recommended for detection of Cdt1 in mice?

Asked by: AbPolly
Thank you for your question. Currently our Cdt1 antibody sc-365305 is only validated for use with human samples. Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Service
Date published: 2017-02-02
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Rated 1 out of 5 by from Not suitable for mouse cells This antibody is not available for IF/ICC with mouse embryonic fibroblasts in our condition (4%PFA 15min fixation followed 1:500 dilution / 4C overnight reaction).
Date published: 2021-08-03
Rated 4 out of 5 by from Cdt1 細胞株でのウエスタンブロッティングで使用しました。希釈倍率1:1000でバンド確認できました。良い抗体だと思います。
Date published: 2021-05-17
Rated 5 out of 5 by from Good specificity in immunofluorescence We used the antibody 1:200 in PBST 0.15% in SKNBE2 cells for immunofluorescence analysis. Cells were fixed with acetone/methanol. The antibody worked very well and we got a specific signal.
Date published: 2020-06-10
Rated 5 out of 5 by from Good antibody Good antibody for WB and we tested it in HCT116 whole cell lysates
Date published: 2018-02-19
Rated 5 out of 5 by from cdt1 This antibody is very good, and the exposure is very good. It is recommended to use
Date published: 2017-08-30
Rated 5 out of 5 by from Great for WBs on extracts from HCT-116 cells! Used at 1:500 in 2% NFDM and got specific signal in response to drug treatment.
Date published: 2017-04-07
Rated 5 out of 5 by from worked for my WB Worked very well for my WB, and trying it for IF now.
Date published: 2017-01-31
Rated 5 out of 5 by from Nice positive western blot We used this antibody for a western blot and got a nice specific band at the correct molecular weight
Date published: 2017-01-03
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