Anti-Cdk9 Antibody (F-6) recommended for detection of Cdk9 of mouse, rat and human origin by WB, IP, IF and ELISA

Anti-Cdk9 Antibody (F-6): sc-376646

Anti-Cdk9 Antibody (F-6) is rated 4.8 out of 5 by 5.
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Datasheets

    • Cdk9 Antibody (F-6) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 343-372 at the C-terminus of Cdk9 of human origin
    • recommended for detection of Cdk9 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine, bovine and porcine
    • See Cdk9 (D-7): sc-13130 for Cdk9 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Cdk9 Antibody (F-6) for WB applications. These reagents are now offered in bundles with Cdk9 Antibody (F-6) (see ordering information below).
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What application is the blocking peptide sc-376646 P appropriate for?

Asked by: cjMara
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-03-01
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Rated 4 out of 5 by from Good for western and co-IP Used at 1:500 dilution to detect endogenous protein in nuclear extract from HEK293T. 5ug antibody per 500ug nuclear extract IPed CDK9 and cyclin T1 is detected with sc-271348 in CDK9 IP lane.
Date published: 2018-08-02
Rated 5 out of 5 by from Nice WB! This antibody gives clean Western blot results at a dilution of 1:200 in HeLa, Jurkat, KNRK, HEK293 and A-431 lysates.
Date published: 2017-02-02
Rated 5 out of 5 by from Good for co-IP It works great for co-IP. I haven't tried it for Western blot.
Date published: 2017-01-16
Rated 5 out of 5 by from Works well for IF applications Works well for IF applications, nuclear and cytoplasmic staining in MeOH fixed HeLa cells. -SCBT QC
Date published: 2015-04-10
Rated 5 out of 5 by from Good antibody for western Good antibody for western, clean background and specific band in HL-60 and NIH/3T3 whole cell lysates. -SCBT QC
Date published: 2014-05-06
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