Anti-Cdk9 Antibody (D-7) recommended for detection of Cdk9 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-Cdk9 Antibody (D-7): sc-13130

Anti-Cdk9 Antibody (D-7) is rated 4.4 out of 5 by 17.
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Datasheets

    • Anti-Cdk9 Antibody (D-7) is a mouse monoclonal IgG2b κ Cdk9 antibody, cited in 85 publications, provided at 200 µg/ml
    • raised against amino acids 204-372 of Cdk9 of human origin
    • Anti-Cdk9 Antibody (D-7) is recommended for detection of Cdk9 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-Cdk9 Antibody (D-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to biotin for WB, IHC(P) and ELISA
    • TransCruz reagent for Gel Supershift and ChIP applications (sc-13130 X, 200 µg/0.1 ml)
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Cdk9 (D-7): sc-13130.
    • m-IgG Fc BP-HRP, m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Cdk9 Antibody (D-7) for WB and IHC(P) applications. These reagents are now offered in bundles with Cdk9 Antibody (D-7) (see ordering information below).
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How has the specificity of this antibody to Cdk9 been validated?

Asked by: AbPolly
Thank you for your question. This specificity of this antibody has been tested by in house QC, including IF data with siRNA knockdown of Cdk9.
Answered by: Technical Support 6
Date published: 2017-01-11

Does this antibody have a blocking peptide associated with it?

Asked by: AbPolly
Thank you for your question. As this antibody was raised against a recombinant protein, unfortunately it is not possible to offer a blocking peptide for it.
Answered by: Technical Support 6
Date published: 2017-01-11
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Rated 4 out of 5 by from Great for western and IP Used at 1:500 dilution to detect endogenous protein in nuclear extract from HEK293T. 5ug antibody per 500ug nuclear extract IPed CDK9; cyclin T1 is detected with sc-271348 in CDK9 IP lane.
Date published: 2018-08-02
Rated 5 out of 5 by from Recommended for western blotting! It detected endogenous CDK9 in lysates from HEK 293T cells (1:500 dilution in 5% milk in TBS-0.1% Tween-20, overnight at 4C).
Date published: 2018-06-18
Rated 1 out of 5 by from Did not work for Chip-seq We received a sample amount of D-7 antibody which we tested in our standard chip-seq experiment pipeline. We did not have sufficient amount to test various buffers. However, the IP with D-7 antibody did not produce any peaks after sequencing.
Date published: 2018-04-10
Rated 5 out of 5 by from CDK9 antibody I bought this item last month,it works pretty well.
Date published: 2017-12-28
Rated 5 out of 5 by from Nice antibody Strong and specific band in Western blotting, publishable quality.
Date published: 2017-12-07
Rated 4 out of 5 by from Good Ab for WB It works good for western-blot in breast cancer cells.
Date published: 2017-08-09
Rated 5 out of 5 by from Good CDK9 antibody This antibody worked well for WB and IP of mouse VSMC proteins.
Date published: 2017-03-09
Rated 4 out of 5 by from Good for Western blot It works for Western blot, but not good for co-IP.
Date published: 2017-01-16
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