Anti-Cdk8 Antibody (D-9) recommended for detection of Cdk8 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-Cdk8 Antibody (D-9): sc-13155

Anti-Cdk8 Antibody (D-9) is rated 4.5 out of 5 by 11.
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Datasheets

    • Anti-Cdk8 Antibody (D-9) is a mouse monoclonal IgG1 κ Cdk8 antibody, cited in 16 publications, provided at 200 µg/ml
    • raised against amino acids 326-464 of Cdk8 of human origin
    • Anti-Cdk8 Antibody (D-9) is recommended for detection of Cdk8 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-Cdk8 Antibody (D-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to TRITC for IF, IHC(P) and FCM
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Cdk8 (D-9): sc-13155.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Cdk8 Antibody (D-9) for WB and IHC(P) applications. These reagents are now offered in bundles with Cdk8 Antibody (D-9) (see ordering information below).
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Basing on the previous question and answer; So, sometimes, 77kDa is possible, right?

Asked by: yu27
Thanks for your follow-up question. Yes, it could be!
Answered by: Tech Service
Date published: 2019-11-19

The question is about molecular weight of CDK8. The first picture is about 77kDa, but the others are 55KDa, which is right? The instruction is 53kDa.

Asked by: yu27
Thank you for your question. The expected molecular weight for CDK8 is around 53-55kDa. Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include phosphorylations, glycosylations or other post-translational modifications.
Answered by: Tech Service
Date published: 2019-11-19

Does this antibody recognise CDK19 as well?

Asked by: edimi
Thank you for your question. This antibody is specific for Cdk8, and does not cross-react with Cdk19.
Answered by: Technical Support
Date published: 2019-08-17

Can Cdk8 (D-9): sc-13155 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Cweed
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, Cdk8 (D-9): sc-13155 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 1 out of 5 by from Didnot work for WB Did not work for western blot in MCF7 breast cancer cell line
Date published: 2017-11-21
Rated 5 out of 5 by from good quality Never meet difficulties use this antibody, very easy operated at 1:500 dilution and got strong band at 53kda.I suggest the loading amount reference to the papers about the expression of the target protein.For high expresion sample loading 30ug is enough.
Date published: 2017-09-10
Rated 5 out of 5 by from VERY GOOD Antiibody I got this antibody for my research and it gives very nice band with no background. We loaded 25 ug of protein and the bands were clear and crisp.
Date published: 2017-09-04
Rated 5 out of 5 by from western blot I used this antibody for western blot and got great results.I blotted cell lysates from primary hippocampal neurons. The signal was not very powerful and no background.
Date published: 2017-08-26
Rated 4 out of 5 by from good It is strong with 1:2000 dilution but has several weak non-specific bands far away in many different types of colon cancer cells.
Date published: 2017-05-12
Rated 5 out of 5 by from good quality This antibody has good quality and specific . I found clear bands, strong signals.
Date published: 2017-05-09
Rated 5 out of 5 by from Versatile Antibody This antibody is well-cited for a variety of applications including WB, IP, IF, and even ELISA. Strong, clear signal with little to no background. Works well in a variety of species including mouse, rat, and human samples.
Date published: 2017-01-30
Rated 5 out of 5 by from Good Cdk8 control antibody for siRNA KO experiment Good Cdk8 control antibody for siRNA KO experiment -SCBT QC
Date published: 2015-04-13
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