Anti-Cdk6 Antibody (B-10) recommended for detection of Cdk6 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-Cdk6 Antibody (B-10): sc-7961

Anti-Cdk6 Antibody (B-10) is rated 4.6 out of 5 by 24.
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Datasheets

    • Anti-Cdk6 Antibody (B-10) is a mouse monoclonal IgG1 κ Cdk6 antibody, cited in 85 publications, provided at 200 µg/ml
    • raised against amino acids 1-326 representing full length Cdk6 of human origin
    • Anti-Cdk6 Antibody (B-10) is recommended for detection of Cdk6 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-Cdk6 Antibody (B-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to TRITC for IF, IHC(P) and FCM
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Cdk6 (B-10): sc-7961.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Cdk6 Antibody (B-10) for WB and IHC(P) applications. These reagents are now offered in bundles with Cdk6 Antibody (B-10) (see ordering information below).
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Can you recommend a paper with protocol for immunohistochemistry staining of Cdk6 Antibody (B-10): sc-7961 monoclonal antibody on human bone marrow tissue? Thank you

Asked by: Regina1
Thank you for your question. We have published immunoperoxidase staining data of formalin fixed, paraffin-embedded human bone marrow tissue showing nuclear and cytoplasmic staining of hematopoietic cells using this mouse monoclonal, Cdk6 Antibody (B-10): sc-7961. Our recommended protocol is provided below. We used sodium citrate buffer (pH 6.0) with heat treatment for the antigen retrieval method. https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-20

Can you recommend a positive control to use with Cdk6 (B-10): sc-7961 monoclonal antibody for Western blot?

Asked by: jenniferc
We recommend HeLa:sc-2200, NCI-H460: sc-364235, HEK293T:sc-45137, Raji: sc-364236, CCRF-CEM: sc-2225 and SCC-4: sc-364363 whole cell lysates as we have great Western blot data showing specific detection of Cdk6 in these cell lines.
Answered by: Technical Support 3
Date published: 2017-01-10
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Rated 5 out of 5 by from WB效果很好 两个月前我申请了该指标试用装 使用1比1000比例稀释 做WB效果很好 条带清晰 性价比高 推荐购买
Date published: 2021-06-15
Rated 4 out of 5 by from Cdk6 (B-10) WB It is suitable for Western Blot in human cell lines
Date published: 2020-11-06
Rated 4 out of 5 by from suitable for WB It is suitable for wb in human cell lines, but it can not apply for IP
Date published: 2019-10-03
Rated 5 out of 5 by from 抗体效果不错,期望买其它抗体也有很好的效果 抗体特异性很好,保存重复使用效果也不会变差。抗体的效价还算可以,总体使用效果好,期望其它的抗体也有较好的效果。
Date published: 2019-07-19
Rated 5 out of 5 by from Very Good! Clean band, no back ground! 1:1000 would in 5% milk work!
Date published: 2018-03-01
Rated 5 out of 5 by from excellent result This antibody was very sensitive to the detection of Cdk6 expression in HeLa cells and I used dilution in 1:1800, especially overnight incubation.
Date published: 2017-11-01
Rated 5 out of 5 by from More applications Cdk6 Antibody has been cited many times,the stains very brightly in cells. Antibody species are relatively complete, other companies do not have the most to be found here, the experimental results are good.
Date published: 2017-10-09
Rated 3 out of 5 by from not too bad dilution ratio1:300 in WB for lovo,sw480,hct116 ,2 days of incubation can get a band .
Date published: 2017-08-22
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