Anti-Cdk5 Antibody (J-3) recommended for detection of Cdk5 of mouse, rat and human origin by WB, IP, IF and ELISA

Anti-Cdk5 Antibody (J-3): sc-6247

Anti-Cdk5 Antibody (J-3) is rated 5.0 out of 5 by 27.
  • y_2021, m_9, d_19, h_20
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.19
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_27
  • loc_en_US, sid_6247, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 83ms
  • REVIEWS, PRODUCT
loading
5
26
4
1
3
0
2
0
1
0

Datasheets

    • Anti-Cdk5 Antibody (J-3) is a mouse monoclonal IgG1 κ Cdk5 antibody, cited in 106 publications, provided at 200 µg/ml
    • raised against amino acids 1-291 representing full length Cdk5 of human origin
    • Anti-Cdk5 Antibody (J-3) is recommended for detection of Cdk5 of mouse, rat and human origin by WB, IP, IF and ELISA
    • Anti-Cdk5 Antibody (J-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to TRITC for IF, IHC(P) and FCM
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Cdk5 (J-3): sc-6247.
    • m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Cdk5 Antibody (J-3) for WB applications. These reagents are now offered in bundles with Cdk5 Antibody (J-3) (see ordering information below).
loading

Have you checked that this antibody is specific for CDK5 and does not recognize other related CDKs including CDK1 and CDK2? Thanks

Asked by: MRou
Thank you for your question. Our mouse monoclonal Cdk5 Antibody (J-3): sc-6247 has been experimentally validated for specificity in Cdk5 CRISPR/Cas9 KO transfected HEK293T whole cell lysates. This data is available on our website. Transfections were performed using our Cdk5 CRISPR/Cas9 KO Plasmid: sc-400161 plus Cdk5 HDR Plasmid: sc-400161-HDR.
Answered by: Technical Support
Date published: 2020-05-08

Can Cdk5 Antibody (J-3): sc-6247 be used for detection of Cdk5 of monkey origin?

Asked by: jenni
Thank you for your inquiry. Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity. We are available by phone, e-mail or chat.
Answered by: Technical Support 3
Date published: 2017-01-09
  • y_2021, m_9, d_20, h_20CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.19
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_2
  • loc_en_US, sid_6247, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 94ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from works perfectly Got this ab as a free sample, works very fine. Clear band at the right molecular weight in mouse brain lysates and SH cells, highly recommended.
Date published: 2021-02-03
Rated 5 out of 5 by from good antibody good antibody, correct band size by western blotting.
Date published: 2017-11-22
Rated 5 out of 5 by from Detects endogenous protein Works well enough for western blot, for endogenous levels of cdk5 in human cell lines and mouse neurons. Used at 1:500.
Date published: 2017-11-21
Rated 5 out of 5 by from Clear and powerful signal Using antibody 1:1000 working in WB and HeLa cells, we used this antibody for many years and always been good signals.
Date published: 2017-11-02
Rated 5 out of 5 by from good signal The antibody can be used for the detection of Cdk5 and provide a good signal, which can help us to study the experime
Date published: 2017-10-20
Rated 5 out of 5 by from Really great We got obtained specific band is very clear and powerful signal with Cdk5 Antibody. Knowledge of the sensitivity and specificity of the antibody leads to their more rational selection.
Date published: 2017-10-18
Rated 5 out of 5 by from great feature work well in WB at 1:100,and the strip is very clear.
Date published: 2017-10-08
Rated 5 out of 5 by from Great This antibody works for mouse brain tissue western blot samples
Date published: 2017-09-06
  • y_2021, m_9, d_19, h_20
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.19
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_27
  • loc_en_US, sid_6247, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 6ms
  • REVIEWS, PRODUCT