Anti-Cdk4 Antibody (F-8) recommended for detection of Cdk4 p34 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-Cdk4 Antibody (F-8): sc-393653

Anti-Cdk4 Antibody (F-8) is rated 5.0 out of 5 by 2.
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Datasheets

    • Cdk4 Antibody (F-8) is a mouse monoclonal IgG1 κ, cited in 2 publications, provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 278-303 at the C-terminus of Cdk4 of mouse origin
    • recommended for detection of Cdk4 p34 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • TransCruz reagent for ChIP application (sc-393653 X, 200 µg/0.1 ml)
    • See Cdk4 (DCS-35): sc-23896 for Cdk4 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Cdk4 Antibody (F-8) for WB and IHC(P) applications. These reagents are now offered in bundles with Cdk4 Antibody (F-8) (see ordering information below).
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What application is the blocking peptide sc-393653 P appropriate for?

Asked by: jenniferc
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Very Specific Western Blot analysis of Cdk4 using this new monoclonal antibody have a very specific single band with no background on several cell lines.
Date published: 2016-12-05
Rated 5 out of 5 by from Good Western blot results of Cdk4 expression Good Western blot results of Cdk4 expression in non-transfected 293T, mouse Cdk4 transfected 293T, SK-BR-3, HeLa and Ramos whole cell lysates.
Date published: 2014-02-12
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