Anti-Cdk4 Antibody (DCS-31) recommended for detection of Cdk4 of mouse, rat and human origin by WB, IP, IF and ELISA

Anti-Cdk4 Antibody (DCS-31): sc-56277

Anti-Cdk4 Antibody (DCS-31) is rated 4.9 out of 5 by 15.
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Datasheets

    • Cdk4 Antibody (DCS-31) is a mouse monoclonal IgG2a κ, cited in 34 publications, provided at 200 µg/ml
    • raised against full length Cdk4 of human origin
    • Anti-Cdk4 Antibody (DCS-31) is recommended for detection of Cdk4 of mouse, rat and human origin by WB, IP, IF and ELISA; non cross-reactive with other Cdk types
    • Anti-Cdk4 Antibody (DCS-31) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Cdk4 (DCS-31): sc-56277.
    • m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Cdk4 Antibody (DCS-31) for WB applications. These reagents are now offered in bundles with Cdk4 Antibody (DCS-31) (see ordering information below).
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I need it for IP experiment. What is the specific protocol for this antibody conjugated to agarose beads?

Asked by: Anonymous
Thank you for your question. You can find our recommended IP protocol by following this link: https://www.scbt.com/resources/protocols/immunoprecipitation-western-blots
Answered by: Tech Support Europe
Date published: 2020-09-07

I work primarily with zebrafish samples. Will this antibody work for me?

Asked by: Cweed
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support 1
Date published: 2017-01-13

I don't have any more of my donkey anti-mouse secondary. What can I use instead?

Asked by: Cweed
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 1
Date published: 2017-01-13
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Rated 4 out of 5 by from Anticorpo Cdk4 Utilizzato in saggi di Western Blot, ha funzionato discretamente.
Date published: 2020-11-06
Rated 5 out of 5 by from Great anitbody! Very specific! no background! used 1:1000 dilution
Date published: 2018-03-01
Rated 5 out of 5 by from Great antibody for western blot The batch of antibody I have worked well on BJAB, HeLa, NIH/3T3, Jurkat and K562 total cell extracts for western Blot. The band is specific. The antibody was diluted 250 times into 5% BSA, into TBST and incubated over-night at 4°C with the membrane.
Date published: 2018-01-04
Rated 5 out of 5 by from Great antibody for IB! This is a great antibody for IB, quite clean signal with little background. Better than sc-23896.
Date published: 2017-11-28
Rated 5 out of 5 by from Good antibody! I like this antibody as it has strong band binding and no background.
Date published: 2017-10-13
Rated 5 out of 5 by from It works excellent in WB This antibody works excellent in WB. Great antibody.
Date published: 2017-09-20
Rated 5 out of 5 by from GOOD FOR IB, IP I tested this antibody to IB(1:100), and IP (2ug/ml lysated protein) in HepG2, PLC/PRF/5, Huh7 and BT-549 cells. It recognizes the endogenous CDK4 protein, and also works for IP.
Date published: 2017-07-10
Rated 5 out of 5 by from WB clear band ,claen background and accurate protein position
Date published: 2017-06-05
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