Anti-Cdk4 Antibody (B-10) recommended for detection of Cdk4 p34 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-Cdk4 Antibody (B-10): sc-166373

Anti-Cdk4 Antibody (B-10) is rated 4.5 out of 5 by 6.
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Datasheets

    • Cdk4 Antibody (B-10) is a mouse monoclonal IgG1 κ, cited in 6 publications, provided at 200 µg/ml
    • raised against amino acids 1-303 representing full length Cdk4 of human origin
    • Anti-Cdk4 Antibody (B-10) is recommended for detection of Cdk4 p34 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-Cdk4 Antibody (B-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Cdk4 (B-10): sc-166373.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Cdk4 Antibody (B-10) for WB and IHC(P) applications. These reagents are now offered in bundles with Cdk4 Antibody (B-10) (see ordering information below).
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I am using Cdk4 (B-10): sc-166373 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Cweed
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Buon funzionamento Comprato l'articolo cinque settimane fa. Funziona bene
Date published: 2021-02-12
Rated 5 out of 5 by from Great antibody for western blot The batch of antibody I have worked well on BJAB, HeLa, NIH/3T3, Jurkat and K562 total cell extracts for western Blot. The band is specific. The antibody was diluted 500 times into 5% BSA, into TBST and incubated over-night at 4°C with the membrane.
Date published: 2018-01-04
Rated 5 out of 5 by from Endogenous expression in NIH/3T3 and HeLa Endogenous expression in NIH/3T3 and HeLa nuclear extracts, strong band at correct size. -SCBT QC
Date published: 2015-04-10
Rated 3 out of 5 by from Worked with transfected lysate by WB with Worked with transfected lysate by WB with little background -SCBT QC
Date published: 2015-03-30
Rated 5 out of 5 by from Works well for IF applications Works well for IF applications, positive in HeLa. -SCBT QC
Date published: 2015-03-19
Rated 5 out of 5 by from IF: Very good nuclear and cytoplasmic staining IF: Very good nuclear and cytoplasmic staining in NIH/3T3 cells. -SCBT QC
Date published: 2013-12-16
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