Anti-Cdk4 Antibody (97) recommended for detection of Cdk4 of mouse, rat and human origin by WB, IP and IF

Anti-Cdk4 Antibody (97): sc-136241

Anti-Cdk4 Antibody (97) is rated 4.0 out of 5 by 1.
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Datasheets

    • Cdk4 Antibody (97) is a mouse monoclonal IgG1, cited in 5 publications, provided at 50 µg/0.5 ml
    • raised against amino acids 1-303 representing full length Cdk4 of rat origin
    • recommended for detection of Cdk4 of mouse, rat and human origin by WB, IP and IF
    • See Cdk4 (DCS-35): sc-23896 for Cdk4 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Cdk4 Antibody (97). This work is in progress.
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I am using Cdk4 (97): sc-136241 for immunofluorescence, what dilution of this antibody should I use?

Asked by: Professor Griffin
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 4 out of 5 by from Single strong band at correct mw Single strong band at correct mw, RSV-3T3 whole cell lysate. -SCBT QC
Date published: 2015-04-10
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