Anti-Cdk4 Antibody (3F121) recommended for detection of Cdk4 of mouse, rat and human origin by WB, IP, IF and IHC(P)

Anti-Cdk4 Antibody (3F121): sc-70831

Anti-Cdk4 Antibody (3F121) is rated 4.7 out of 5 by 3.
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Datasheets

    • Cdk4 Antibody (3F121) is a mouse monoclonal IgG1 κ, cited in 12 publications, provided at 200 µg/ml
    • raised against full length recombinant human Cdk4
    • recommended for detection of Cdk4 of mouse, rat and human origin by WB, IP, IF and IHC(P)
    • See Cdk4 (DCS-35): sc-23896 for Cdk4 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Cdk4 Antibody (3F121) for WB and IHC(P) applications. These reagents are now offered in bundles with Cdk4 Antibody (3F121) (see ordering information below).
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I am using Cdk4 (3F121): sc-70831 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Trav11
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from This antibody works in various human cell This antibody works in various human cell lines by WB -SCBT Publication Review
Date published: 2015-04-13
Rated 4 out of 5 by from Works well for WB with in HeLa and Ramos Works well for WB with in HeLa and Ramos whole cell lysate. Some background bands
Date published: 2015-01-22
Rated 5 out of 5 by from Worked with transfected lysate by WB Worked with transfected lysate by WB -SCBT QC
Date published: 2013-09-27
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