Anti-Cdk4 Antibody (0.N.199) recommended for detection of Cdk4 of mouse, rat and human origin by WB, IP, IF and IHC(P)

Anti-Cdk4 Antibody (0.N.199): sc-70832

Anti-Cdk4 Antibody (0.N.199) is rated 4.7 out of 5 by 3.
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Datasheets

    • Cdk4 Antibody (0.N.199) is a mouse monoclonal IgG1 κ, cited in 16 publications, provided at 200 µg/ml
    • raised against full length recombinant human Cdk4
    • recommended for detection of Cdk4 of mouse, rat and human origin by WB, IP, IF and IHC(P)
    • See Cdk4 (DCS-35): sc-23896 for Cdk4 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Cdk4 Antibody (0.N.199). This work is in progress.
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Can Cdk4 (0.N.199): sc-70832 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Cweed
Thank you for your question. Yes, Cdk4 (0.N.199): sc-70832 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-28
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Rated 5 out of 5 by from Publishable Western blot results using HepG2 Publishable Western blot results using HepG2, HHCC, SMMC7721, QZG and AD-293 cell lines -SCBT Publication Review
Date published: 2015-04-13
Rated 4 out of 5 by from Good specificity and signal strength with Good specificity and signal strength with HeLa and Ramos. Some background bands -SCBT QC
Date published: 2015-01-30
Rated 5 out of 5 by from Worked with transfected lysate by WB Worked with transfected lysate by WB -SCBT QC
Date published: 2014-03-14
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