Anti-Cdk2 Antibody (D-12) recommended for detection of Cdk2 of mouse, rat and human origin by WB, IP, IF and ELISA

Anti-Cdk2 Antibody (D-12): sc-6248

Anti-Cdk2 Antibody (D-12) is rated 4.8 out of 5 by 43.
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Datasheets

    • Cdk2 Antibody (D-12) is a mouse monoclonal IgG1 κ, cited in 414 publications, provided at 200 µg/ml
    • raised against amino acids 1-298 representing full length Cdk2 of human origin
    • Anti-Cdk2 Antibody (D-12) is recommended for detection of Cdk2 of mouse, rat and human origin by WB, IP, IF and ELISA
    • Anti-Cdk2 Antibody (D-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to either TRITC or Alexa Fluor® 405 for IF, IHC(P) and FCM
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Cdk2 (D-12): sc-6248.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Cdk2 Antibody (D-12) for WB applications. These reagents are now offered in bundles with Cdk2 Antibody (D-12) (see ordering information below).
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What is the recommended secondary reagent to be used with this Cdk (D-12) mouse monoclonal primary by Western blot?

Asked by: jenni
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Santa Cruz TS
Date published: 2016-12-20

Can I use Cdk2 Antibody (D-12) mouse monoclonal antibody for immunohistochemical staining of mouse tissue?

Asked by: jenni
Yes. We recommend using a directly conjugated mouse monoclonal primary antibody. Cdk2 (D-12) mouse monoclonal antibody is currently available conjugated to HRP, FITC, TRITC, PE, AF405, AF488 and AF647
Answered by: Santa Cruz TS
Date published: 2016-12-20
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Rated 1 out of 5 by from 抗体非特异性不好 WB目的条带上有杂带,而且IF非特异性信号特别多,无法区分WT和Cdk2-KO,根本不像展示的那么好!
Date published: 2019-11-23
Rated 5 out of 5 by from good 1/500 dilution was used for WB, and the results are good.
Date published: 2018-12-24
Rated 5 out of 5 by from Great product!! It's a great product for me to analyze Western blotting.
Date published: 2018-04-22
Rated 5 out of 5 by from Great features! I bought this a month ago and am so happy that I had gotten a perfect picture of Western blot analysis.
Date published: 2017-12-12
Rated 5 out of 5 by from Great features! I bought this a month ago and am so happy that I had gotten a perfect picture of Western blot analysis.
Date published: 2017-12-12
Rated 5 out of 5 by from Great antibody for IB! This antibody is great for IB, same result compared to CST #2546, but much cheaper.
Date published: 2017-11-28
Rated 5 out of 5 by from Great! the antibody is good at several human lung cancer cell line at a dilution of 1:1000.
Date published: 2017-11-07
Rated 5 out of 5 by from Works well This antibody showed a unique, powerful band in Western blot studies of rat liver tissue.
Date published: 2017-10-27
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