Anti-Cdk2 Antibody (AN4.3) recommended for detection of Cdk2 of mouse, rat and human origin by WB and IP

Anti-Cdk2 Antibody (AN4.3): sc-53220

Anti-Cdk2 Antibody (AN4.3) is rated 4.7 out of 5 by 3.
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Datasheets

    • Cdk2 Antibody (AN4.3) is a mouse monoclonal IgG2a κ, cited in 9 publications, provided at 200 µg/ml
    • raised against human recombinant Cdk2
    • recommended for detection of Cdk2 of mouse, rat and human origin by WB and IP
    • See Cdk2 (D-12): sc-6248 for Cdk2 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Cdk2 Antibody (AN4.3). This work is in progress.
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For Western Blot, is it recommended to use denatured or non-denatured conditions with Cdk2 (AN4.3): sc-53220 monoclonal antibody?

Asked by: AbPolly
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
Answered by: Technical Support
Date published: 2017-02-27
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Rated 4 out of 5 by from Worked with transfected lysate by WB Worked with transfected lysate by WB -SCBT QC
Date published: 2015-04-13
Rated 5 out of 5 by from The antibody works well for Western blotting The antibody works well for Western blotting detecting endogenous Cdk2 from A-431 cells. -SCBT QC
Date published: 2015-03-17
Rated 5 out of 5 by from Good specificity and signal strength in K Good specificity and signal strength in K-562, HeLa and NAMALWA whole cell lysates. -SCBT QC
Date published: 2014-05-18
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