Anti-Cdk2 Antibody (55) recommended for detection of Cdk2 of mouse, rat, human and canine origin by WB, IP and IF

Anti-Cdk2 Antibody (55): sc-136191

Anti-Cdk2 Antibody (55) is rated 4.0 out of 5 by 2.
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Datasheets

    • Cdk2 Antibody (55) is a mouse monoclonal IgG2a, cited in 5 publications, provided at 50 µg/0.5 ml
    • raised against amino acids 109-298 of Cdk2 of human origin
    • recommended for detection of Cdk2 of mouse, rat, human and canine origin by WB, IP and IF
    • See Cdk2 (D-12): sc-6248 for Cdk2 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Cdk2 Antibody (55). This work is in progress.
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I am using Cdk2 (55): sc-136191 for immunofluorescence, what dilution of this antibody should I use?

Asked by: Professor Griffin
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by from Produced a clear band with Jurkat whole cell Produced a clear band with Jurkat whole cell lysate. -SCBT QC
Date published: 2015-03-31
Rated 3 out of 5 by from Worked with transfected lysate by WB Worked with transfected lysate by WB -SCBT QC
Date published: 2013-11-09
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