Anti-Cdk1/Cdk2 Antibody (AN21.2) recommended for detection of Cdk1/Cdk2 of mouse, rat and human origin by WB, IP, IF and IHC(P)

Anti-Cdk1/Cdk2 Antibody (AN21.2): sc-53219

Anti-Cdk1/Cdk2 Antibody (AN21.2) is rated 4.8 out of 5 by 15.
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Datasheets

    • Anti-Cdk1/Cdk2 Antibody (AN21.2) is a mouse monoclonal IgG2a κ Cdk1/Cdk2 antibody, cited in 56 publications, provided at 200 µg/ml
    • raised against human recombinant Cdk2
    • Anti-Cdk1/Cdk2 Antibody (AN21.2) is recommended for detection of Cdk1/Cdk2 of mouse, rat and human origin by WB, IP, IF and IHC(P)
    • Anti-Cdk1/Cdk2 Antibody (AN21.2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Cdk1/Cdk2 (AN21.2): sc-53219.
    • m-IgGκ BP-HRP is the preferred secondary detection reagent for Cdk1/Cdk2 Antibody (AN21.2) for WB and IHC(P) applications. This reagent is now offered in a bundle with Cdk1/Cdk2 Antibody (AN21.2) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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Is this antibody detect both CDK1 and CDK2? In the above CITATIONS, it seems people used it for detecting CDK1, can you explain why?

Asked by: XW Song
Thank you for your question. This mouse monoclonal antibody was raised against human recombinant Cdk2. The AN21.2 clone is useful as a general Cdk antibody since it reacts with both Cdk1 and Cdk2. These are closely related proteins with a high degree of sequence homology, and useful markers for proliferation.
Answered by: Technical Support
Date published: 2019-01-26

Hi, I want to receive a FREE 10 µg sample of Cdk1/Cdk2 (AN21.2): sc-53219.

Asked by: Qi Wang
Thank you for your question. We would be happy to send you a sample of this antibody, sc-53219. Please contact Technical Service by phone, (800)-457-3801 option 2, email <[email protected]>, or by live chat directly on our website, www.scbt.com, and we would be happy to send you a sample.
Answered by: Tech Service
Date published: 2017-06-24

Can this antibody be used to stain mouse tissue and cells in IHC and IF?

Asked by: AbPolly
Thank you for your question. Yes. We recommend using a directly conjugated mouse monoclonal primary antibody. This mouse monoclonal antibody is currently available conjugated to HRP, PE, FITC, AF488 and AF647.
Answered by: Technical Support 12
Date published: 2017-01-20

Is there a blocking peptide associated with this antibody?

Asked by: AbPolly
Thank you for your question. This antibody is raised against a recombinant protein and not a short synthetic peptide, so we are unable to offer a blocking peptide for it.
Answered by: Technical Support 14
Date published: 2017-01-19
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Rated 5 out of 5 by from Great product!! It's a great product for me to analyze Western blotting.
Date published: 2018-04-22
Rated 5 out of 5 by from Really my favorate. I like this antibody because of its strong and specific binding.
Date published: 2017-10-13
Rated 4 out of 5 by from Good for WB it works good for WB in our lab, not a bad choice.
Date published: 2017-09-18
Rated 5 out of 5 by from nice antibody very good antibody can be used in mouse ES cells and can be detect only one band in arm band.
Date published: 2017-08-03
Rated 5 out of 5 by from Nice Western Blot data of SNAT2 expression in non Produced beautiful immunofluorescence membrane and cytoplasmic vesicles staining in methanol-fixed HeLa cells.Nice Western Blot data of SNAT2 expression in non-transfected and human SNAT2 transfected 293T whole cell lysates.
Date published: 2017-07-10
Rated 5 out of 5 by from The effect is very good. Use gamma-GCSc antibody technique for detecting of rat was established successfully and can be used for analyzing samples from rat, with a precise position effect and very powerful signal.
Date published: 2017-07-10
Rated 5 out of 5 by from excellent antibody for westerns diluted at 1000X and used with the Kappa-binding HRP as secondary, and the blot turned out perfect with no background.
Date published: 2017-06-16
Rated 4 out of 5 by from good antibody this antibody works good for human gastric cancer cellline
Date published: 2017-05-18
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