Anti-Cdc6 Antibody (D-1) recommended for detection of Cdc6 of human origin by WB, IP, IF and ELISA

Anti-Cdc6 Antibody (D-1): sc-13136

Anti-Cdc6 Antibody (D-1) is rated 4.3 out of 5 by 9.
  • y_2021, m_11, d_29, h_18
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.20
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_9
  • loc_en_US, sid_13136, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 115ms
  • REVIEWS, PRODUCT
loading
5
5
4
2
3
0
2
0
1
1

Datasheets

    • Cdc6 Antibody (D-1) is a mouse monoclonal IgG1 κ, cited in 10 publications, provided at 200 µg/ml
    • raised against amino acids 257-560 of Cdc6 of human origin
    • Cdc6 Antibody (D-1) is recommended for detection of Cdc6 of human origin by WB, IP, IF and ELISA
    • Anti-Cdc6 Antibody (D-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Cdc6 (D-1): sc-13136.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Cdc6 Antibody (D-1) for WB applications. These reagents are now offered in bundles with Cdc6 Antibody (D-1) (see ordering information below).
loading

What is the recommended fixation method for immunofluorescence staining with Cdc6 (D-1): sc-13136 monoclonal antibody?

Asked by: DefinitelyNotMatt
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
  • y_2021, m_11, d_29, h_18CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.20
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_1
  • loc_en_US, sid_13136, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 127ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Great antibody for Western blot This antibody performs very well for Western blot for multiple human cell lines.
Date published: 2020-01-05
Rated 1 out of 5 by from CDC6 Ab The antibody didn´t give me any signal using HCT116 nuclear extracts performed by standard WB procedure that worked for another Santa Cruz antibodies (for example sc-9964 – also against CDC6).
Date published: 2018-07-23
Rated 5 out of 5 by from Excellent antibody! Published for ChIP. This monoclonal Cdc6 Antibody (D-1) has excellent in-house data for Western blot and IF, and it has been published for ChIP (see PMID: 26872786).
Date published: 2017-02-07
Rated 5 out of 5 by from good antibody good antibody, used in WB on human samples
Date published: 2016-07-25
Rated 4 out of 5 by from This antibody works well and can be used This antibody works well and can be used for IP and Western Blot with human HeLa, T98G, U2-OS, HEK 293T, H1299 cell lines. -SCBT Publication Review
Date published: 2015-04-29
Rated 5 out of 5 by from Works well in Western Blot application with Works well in Western Blot application with the human HEF cell line. -SCBT Publication Review
Date published: 2015-03-18
Rated 5 out of 5 by from Strong band at correct molecular weight observed Strong band at correct molecular weight observed by Western Blot with HeLa nuclear extract. -SCBT QC
Date published: 2015-01-19
Rated 5 out of 5 by from Produced good quality flow cytometry data Produced good quality flow cytometry data in HeLa and S3 cells. -SCBT Publication Review
Date published: 2015-01-09
  • y_2021, m_11, d_29, h_18
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.20
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_9
  • loc_en_US, sid_13136, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 14ms
  • REVIEWS, PRODUCT