Anti-Cdc37 Antibody (E-4) recommended for detection of Cdc37 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-Cdc37 Antibody (E-4): sc-13129

Anti-Cdc37 Antibody (E-4) is rated 4.9 out of 5 by 11.
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Datasheets

    • Anti-Cdc37 Antibody (E-4) is a mouse monoclonal IgG2b κ Cdc37 antibody, cited in 38 publications, provided at 200 µg/ml
    • raised against amino acids 108-378 of Cdc37 of human origin
    • Anti-Cdc37 Antibody (E-4) is recommended for detection of Cdc37 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-Cdc37 Antibody (E-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to TRITC for IF, IHC(P) and FCM
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Cdc37 (E-4): sc-13129.
    • m-IgG Fc BP-HRP, m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Cdc37 Antibody (E-4) for WB and IHC(P) applications. These reagents are now offered in bundles with Cdc37 Antibody (E-4) (see ordering information below).
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Hi I would like to use this antibody (Cdc37 (E-4)) for immunoprecipitation. Has anyone tested it for this? How much antibody was used?

Asked by: Henriette
Thank you for your question. This antibody is recommended and warrantied for Immunoprecipitation use, and there are several publications in that application. Here is a link to our recommended protocol: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots If you have any further questions, please contact Technical Service by phone, (800)-457-3801 option 2, email <s[email protected]>, or by live chat directly on our website, www.scbt.com.
Answered by: Tech Service
Date published: 2017-08-28

I am using Cdc37 (E-4): sc-13129 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: cjMara
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 5
Date published: 2017-01-25
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Rated 5 out of 5 by from Excellent in WB Excellent in WB Dilution 1:1000 clear and specific band
Date published: 2021-02-25
Rated 4 out of 5 by from Great antibody This antibody works well for IP and also WB. Thank you, Santa Cruz.
Date published: 2018-03-25
Rated 5 out of 5 by from Good antibody This antibody worked very good for WB and IP. WB diluted 1:1000 in 293t cell lysate.
Date published: 2017-09-28
Rated 5 out of 5 by from very helpful This antibody is very helpful for analyisis cdc37 in HepG2 cell, I got clear and strong signal band at 50kda.This is the target band, no background by dilution 1;600.
Date published: 2017-08-26
Rated 5 out of 5 by from Pretty good Good specificity in WB in rat testis tissue, we usually use the dilution 1:300.
Date published: 2017-03-18
Rated 5 out of 5 by from versitile antibody Used this antibody for IHC(P), IF and WB in multiple samples, which produced nice and specific signal
Date published: 2017-01-17
Rated 5 out of 5 by from Good cytoplasmic staining of formalin fixed Good cytoplasmic staining of formalin fixed glandular cells. -SCBT Publication Review
Date published: 2015-04-11
Rated 5 out of 5 by from Strong WB band in Jurkat Strong WB band in Jurkat, CTLL-2 and KNRK whole cell lysates. -SCBT QC
Date published: 2015-04-11
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