Anti-Cdc34B Antibody (C-4) recommended for detection of Cdc34B of mouse, rat and human origin by WB, IP, IF and ELISA

Anti-Cdc34B Antibody (C-4): sc-376427

Anti-Cdc34B Antibody (C-4) is rated 5.0 out of 5 by 2.
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Datasheets

    • Cdc34B Antibody (C-4) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 188-238 mapping at the C-terminus of Cdc34B of human origin
    • recommended for detection of Cdc34B of mouse, rat and human origin by WB, IP, IF and ELISA
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Cdc34B Antibody (C-4) for WB applications. These reagents are now offered in bundles with Cdc34B Antibody (C-4) (see ordering information below).
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What is the recommended fixation method for immunofluorescence staining with Cdc34B (C-4): sc-376427 monoclonal antibody?

Asked by: jerojero
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from Good cytoplasmic localization in methanol Good cytoplasmic localization in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-02-18
Rated 5 out of 5 by from Worked well in WB in Jurkat and C6 whole Worked well in WB in Jurkat and C6 whole cell lysates. -SCBT QC
Date published: 2013-09-16
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