Anti-Cdc27 Antibody (C-4) recommended for detection of Cdc27 of mouse, rat and human origin by WB, IP, IF, IHC(P), FCM and ELISA

Anti-Cdc27 Antibody (C-4): sc-13154

Anti-Cdc27 Antibody (C-4) is rated 4.8 out of 5 by 8.
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Datasheets

    • Cdc27 Antibody (C-4) is a mouse monoclonal IgG2a κ, cited in 14 publications, provided at 200 µg/ml
    • raised against amino acids 1-300 of Cdc27 of human origin
    • Anti-Cdc27 Antibody (C-4) is recommended for detection of Cdc27 of mouse, rat and human origin by WB, IP, IF, IHC(P), FCM and ELISA
    • Anti-Cdc27 Antibody (C-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Cdc27 (C-4): sc-13154.
    • m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Cdc27 Antibody (C-4) for WB and IHC(P) applications. These reagents are now offered in bundles with Cdc27 Antibody (C-4) (see ordering information below).
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Can Cdc27 (C-4): sc-13154 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: DefinitelyNotMatt
Thank you for your question. Yes, Cdc27 (C-4): sc-13154 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-25
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Rated 5 out of 5 by from good product This is a good product, pretty clean with only one non-specific band on U2OS cell line WB.
Date published: 2017-12-26
Rated 5 out of 5 by from Excellent Result I used Cdc27 for my WB. It gave me excellent result. I got very nice strong bands.
Date published: 2017-09-29
Rated 5 out of 5 by from satisfactorily We have data demonstrating that Cdc16 and Cdc27 are components of APC, and that ubiquitination of cyclin B leads to the degradation of the cyclin B / Cdk complex. It is very gratifying that the results of our experiments have been satisfactorily achieved
Date published: 2017-02-02
Rated 5 out of 5 by from Good results obtained by immunofluorescence Good results obtained by immunofluorescence when staining for mouse testis. -SCBT Publication Review
Date published: 2015-04-10
Rated 4 out of 5 by from This antibody was found to be suitable for This antibody was found to be suitable for use in flow cytometry applications using fixed and permeabolized K-562 cells. -SCBT QC
Date published: 2015-04-08
Rated 5 out of 5 by from Great antibody for human and rat immunoprecipitation, published using human and mouse cerebellar branules, HeLa and HEK293 cell lines. -SCBT Publication Review
Date published: 2015-03-01
Rated 5 out of 5 by from Strong band at correct molecular weight observed Strong band at correct molecular weight observed by Western Blot with K-562 whole cell lysate. -SCBT QC
Date published: 2015-02-02
Rated 4 out of 5 by from Good nuclear staining of surface epithelial Good nuclear staining of surface epithelial cells of formalin fixed paraffin embedded (PPFE) human esophagus tissue. -SCBT QC
Date published: 2014-11-13
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